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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
COL-10, monoclonal
分子量
antigen 60 kDa (in denatured-reduced preparations)
包含
15 mM sodium azide
种属反应性
deer, human, pig
技术
dot blot: suitable
immunocytochemistry: 1:1,000 using HT 1080 human fibrosarcoma cells
western blot: suitable
同位素/亚型
IgM
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... COL10A1(1300)
一般描述
抗X型胶原蛋白单克隆抗体(小鼠IgM同种型)源自COL-10杂交瘤,该杂交瘤是通过将小鼠骨髓瘤细胞与用X型猪胶原蛋白免疫的BALB/c小鼠的脾细胞融合而产生的。X型胶原蛋白具有相似的结构域结构与VIII型胶原蛋白:50 kDa的中央三螺旋(COL1)结构域两侧是N端(NC2)和C端(NC1)非三螺旋结构域。
胶原蛋白是由三种多肽组成的细胞外糖蛋白,它们混合形成三重螺旋。X型胶原蛋白是在长骨,椎骨和肋骨生长区的胎儿肥大软骨中发现的59 kDα1(X)链的同源三聚体,而在成年人中,它也存在于甲状软骨中。单克隆抗胶原X型抗体可用于细胞培养物的组织学和免疫组织化学评估。它也可用于研究特定的差异组织表达。
免疫原
纯化的猪X型胶原。
应用
小鼠X型抗胶原蛋白单克隆抗体可用于
- 酶联免疫吸附测定(ELISA)
- 斑点印迹
- 免疫印迹和
- 免疫组化。
生化/生理作用
X型胶原是肥大软骨细胞的产物。X型胶原是非纤维状的,但形成与软骨胶原有关的细的细胞周细丝。它与基质蛋白(如连接蛋白V、软骨钙黄绿素、胶原II和蛋白聚糖)以及Ca2+相互作用。它在软骨内骨化过程中充当避免局部衰竭的支架,并且在软骨矿化中也起作用。人α1(X)胶原基因的NC1编码域中的突变与Schmid干骺端软骨形成有关。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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