产品名称
单克隆抗-胶原，X型 小鼠抗, clone COL-10, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
COL-10, monoclonal
mol wt
antigen 60 kDa (in denatured-reduced preparations)
contains
15 mM sodium azide
species reactivity
deer, human, pig
technique(s)
dot blot: suitable
immunocytochemistry: 1:1,000 using HT 1080 human fibrosarcoma cells
western blot: suitable
isotype
IgM
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... COL10A1(1300)
Application
小鼠X型抗胶原蛋白单克隆抗体可用于
- 酶联免疫吸附测定（ELISA）
- 斑点印迹
- 免疫印迹和
- 免疫组化。
Biochem/physiol Actions
X型胶原是肥大软骨细胞的产物。X型胶原是非纤维状的，但形成与软骨胶原有关的细的细胞周细丝。它与基质蛋白（如连接蛋白V、软骨钙黄绿素、胶原II和蛋白聚糖）以及Ca2+相互作用。它在软骨内骨化过程中充当避免局部衰竭的支架，并且在软骨矿化中也起作用。人α1（X）胶原基因的NC1编码域中的突变与Schmid干骺端软骨形成有关。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
抗X型胶原蛋白单克隆抗体（小鼠IgM同种型）源自COL-10杂交瘤，该杂交瘤是通过将小鼠骨髓瘤细胞与用X型猪胶原蛋白免疫的BALB/c小鼠的脾细胞融合而产生的。X型胶原蛋白具有相似的结构域结构与VIII型胶原蛋白：50 kDa的中央三螺旋（COL1）结构域两侧是N端（NC2）和C端（NC1）非三螺旋结构域。
胶原蛋白是由三种多肽组成的细胞外糖蛋白，它们混合形成三重螺旋。X型胶原蛋白是在长骨，椎骨和肋骨生长区的胎儿肥大软骨中发现的59 kDα1（X）链的同源三聚体，而在成年人中，它也存在于甲状软骨中。单克隆抗胶原X型抗体可用于细胞培养物的组织学和免疫组织化学评估。它也可用于研究特定的差异组织表达。
Immunogen
纯化的猪X型胶原。
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
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Ahmad PJ, et al.
The American Journal of Pathology, 175(6), 2686-2696 (2009)
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Iranian journal of basic medical sciences, 16(11), 1163-1169 (2014-02-05)
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