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C7901

Sigma-Aldrich

Chelex ® 100 钠形式

50-100 mesh (dry)

别名:

Iminodiacetic Acid Resin

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About This Item

CAS号:
11139-85-8
MDL编号:
MFCD00163980
UNSPSC代码:
23151817
NACRES:
NA.56

表单

beads

质量水平

200

基质

1% cross-linked polystyrene
 cross-linked polystyrene beads

基质活性基团

iminodiacetic acid (sodium form)
paired iminodiacetate groups

基质附着

through methyl group to aromatic rings

粒径

50-100 mesh (dry)

pH值（酸碱度）

4-14

容量

≥0.3 meq/mL binding capacity
 0.6 meq/g binding capacity (heavy metal ions)

应用

Chelex® 100 sodium form已用于：
  • 从蜜蜂样本中提取DNA
  • 去除香烟烟雾提取物（CSE）并研究铁调素在小鼠肺组织中表达的影响
  • 从细菌培养物中提取DNA

Chelex已用于亲和层析、蛋白质层析、螯合树脂、亲和树脂以及核心生物试剂。

生化/生理作用

Chelex® 100树脂是含有配对亚氨基二乙酸离子（在结合多价金属离子过程中用作螯合基团）的苯乙烯-二乙烯基苯共聚物。它归为弱阳离子型树脂。Chelex作为一种螯合树脂，用于从法医样本（如头发、血迹卡和口腔拭子）中提取DNA。

其他说明

钠形式产品可进行高压蒸汽灭菌。最高工作温度75°C ，不溶于水。

法律信息

Chelex is a registered trademark of Bio-Rad Laboratories, Inc.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000415160
0000415160
0000414956
0000414956
0000410726

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What solvent should I use to swell the Product C7901, Chelex 100 at the beginning?

    We recommend using a good grade of water (deionized or distilled).

  4. My Chelex 100 (Product C7901) in my column dried out.  Can I still use it?

    The resin may be rehydrated and used, as long as it remains white.  If the color of the re-swollen resin is yellow, do not use it.

  5. I want to use Product C7901, Chelex 100, to make a metal-free solution.  However, the water wash from the Chelex resin is very basic.  How can I fix this?

    A suspension of Chelex 100 (sodium form) in water typically has a pH of 10-11. This pH can be slowly lowered by extended water washing, or by using a buffer.  The sodium form at pH 6.3 can be obtained by rinsing with at least 4 bed volumes of 0.5 N sodium acetate buffer, followed by 5 bed volumes of water. This is a more common method for making metal-free distilled water.

  6. What is the particle size of the resin in Product C7901, Chelex 100?

    The particle size for this resin is 45-165 microns. The mean particle size is 90 microns.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

T P Ryan et al.
Journal of biochemical toxicology, 8(1), 33-39 (1993-03-01)
The effects of transition metals on nonenzymatic and ceruloplasmin catalyzed epinephrine oxidation were investigated by studying rates of epinephrine oxidation in purified buffers and in the presence of metal chelating agents. We found that epinephrine does not "autoxidize" in sodium
Vit Molva et al.
Insect science, 27(2), 266-275 (2018-08-14)
The interaction of house dust mites (HDM) and microorganisms is the key factor in the survival of these mites in human-made environments. Spent growth medium (SPGM) provides the rest of the diet, along with dead mite bodies and microorganisms. SPGM
Detection and quantification of Melissococcus plutonius in honey bee workers exposed to European foulbrood in Czechia through conventional PCR, qPCR, and barcode sequencing
Sopko B, et al.
Journal of Apicultural Research, 59(4), 503-514 (2020)
Methods for extracting genomic DNA from whole blood samples: current perspectives
Chacon Cortes D, et al.
Journal of Biosciences, 2014(2), 1-9 (2014)
Simultaneous detection of Escherichia coli O175: H7, Salmonella spp., and Listeria monocytogenes by multiplex PCR
Germini A, et al.
Food Control, 20(8), 733-738 (2009)
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