产品名称
单克隆抗-胶原蛋白,III型 小鼠抗, clone FH-7A, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
FH-7A, monoclonal
mol wt
antigen 70 kDa
contains
15 mM sodium azide
species reactivity
human, rat
technique(s)
dot blot: suitable
immunohistochemistry (frozen sections): 1-4,000 using frozen sections of human skin
indirect ELISA: suitable
western blot: suitable using a denatured-reduced preparation
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... COL3A1(1281)
rat ... Col3a1(84032)
General description
单克隆抗胶原蛋白III型(小鼠IgG1同种型)分离自FH-7A 杂交瘤,其通过融合小鼠骨髓瘤细胞和人III型胶原蛋白免疫的BALB /c小鼠的脾细胞产生。
胶原蛋白是一种纤维蛋白,存在于所有脊椎动物的细胞外骨架中。胶原蛋白III是一种分子量300 kD的分子,属于纤维胶原蛋白类,主要存在于皮肤、血管、肝脏、胎盘、舌头和胸腺。这种胶原蛋白与多种遗传疾病直接或间接相关,如 Ehlers-Danlos IV 型综合征。 III 型抗胶原蛋白单克隆抗体可用于测定总蛋白匀浆液中的间质胶原间质胶原蛋白I和III的含量。它还用于研究III型胶原蛋白在正常和肿瘤性组织中的免疫定位。小鼠单克隆抗III型胶原蛋白抗体可与天然和变性的III型胶原蛋白发生特异性反应,但是不与I、II、IV、V、VI和X型胶原蛋白反应。
Application
小鼠源抗III型胶原蛋白单克隆抗体可用于酶联免疫吸附测定 (ELISA)和免疫组化。
抗 III 型抗胶原蛋白单克隆抗体可用于免疫荧光和蛋白质印迹。
Biochem/physiol Actions
抗III型胶原单克隆抗体可与天然和变性的III型胶原发生特异性反应。它无法识别I、II、IV、V、VI和X型胶原。
这种胶原蛋白与多种遗传疾病直接或间接相关,如 Ehlers-Danlos IV 型综合征。 III 型抗胶原蛋白单克隆抗体可用于测定总蛋白匀浆液中的间质胶原间质胶原蛋白I和III的含量。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途,或人类或动物任何类型的消费或使用。
Immunogen
人III型胶原
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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