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Merck
CN

C7642

Sigma-Aldrich

伴刀豆球蛋白A 来源于洋刀豆 (刀豆)

FITC conjugate, Type IV, lyophilized powder

别名:

ConA

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About This Item

UNSPSC代码:
12352202
NACRES:
NA.32

生物来源

Canavalia ensiformis

质量水平

偶联物

FITC conjugate

类型

Type IV

形式

lyophilized powder

组成

Protein, 5-15% biuret

标记范围

3-6 mol FITC per mol protein

杂质

Carbohydrate, essentially free

储存温度

−20°C

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应用

用于荧光检测含有 α−-D-甘露糖或 α−-D-葡萄糖的糖蛋白

生化/生理作用

刀豆素 A 无血型特异性,但对末端 α-D-甘露糖基和 α-D-葡糖基残基具有亲和力。需要 Ca2+ 和 Mn2+ 离子存在才能显示活性。刀豆素 A 在 pH 6.5 或更低的条件下解离为二聚体。pH 在 5.8 至 7.0 之间时,刀豆素 A 以四聚体形式存在;pH 高于 7.0 时,形成更高的聚合体。根据聚合程度不同,刀豆素 A 可能显示出具有促有丝分裂的活性。琥珀酰化可以生成一种能够在 pH 高于 5.6 时仍保持二聚体形式的活性二聚体。

包装

包装尺寸基于蛋白质含量

外形

含有磷酸盐缓冲盐和 NaCl 的冻干粉末

分析说明

在报道中,凝集活性以μg/ml表示,并且是在含有1mg/mL Ca 2+和Mn 2+的磷酸盐缓冲盐水(pH 6.8)的系列稀释液中进行测定的。该活性是在25℃孵育1小时后,对2%人红细胞悬浮液进行凝集所需的最低浓度。

象形图

Exclamation mark

警示用语:

Warning

危险声明

预防措施声明

危险分类

Skin Sens. 1

WGK

WGK 3

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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