推荐产品
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen 28 kDa
种属反应性
rat, chicken, human
增强验证
independent
Learn more about Antibody Enhanced Validation
技术
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using sections of rat, human or chicken cerebellum sections
microarray: suitable
western blot: 1:1,000 using recombinant rat calbindin-D-28K
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... CALB1(793)
mouse ... Calb1(12307)
rat ... Calb1(83839)
一般描述
Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin), is a highly conserved 28 kDa calcium binding protein, with broad tissue distribution. Itbelongs , to a family of low molecular weight calcium-binding proteins (CaBPs). Calbindin-D-28K is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial cells.
免疫原
synthetic peptide corresponding to the C-terminal region of rat calbindin-D-28K (amino acids 185-199). This sequence is identical in the corresponding human, mouse and bovine calbindin-D-28K sequences and is highly conserved (single amino acid substitution) in chicken and frog calbindin-D-28K.
应用
Anti-Calbindin-D-28K (KD-15) antibody produced in rabbit has been used in:
- immunocytochemistry
- western blotting
- microarray
- immunohistochemistry
生化/生理作用
Calbindin 1 (CALB) is a calcium-binding protein. The protein may play a role in development of Purkinje cells found in heterotopias and cerebellar dysgenesias. It also acts as a very important component of intracellular homeostasis in cerebellar neurons. The progressive decrease of calbindin content in the Purkinje cells undergoes degeneration and death during paraneoplastic changes in the cerebellum. Calbindin effects on Parkinson′s disease (PD) risk displays population specificity.
Calbindin-D-28K is an important neuroanatomical marker. Despite its broad tissue distribution, it exhibits a cell-type-specific expression pattern. It has been immunocytochemically localized in selected cells in hippocampus, where it is thought to buffer intracellular calcium, or transport intramembranous calcium.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
分析说明
Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min. at RT or 37 °C) improves immunohistochemical staining.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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分析证书(COA)
Lot/Batch Number
Janelle M P Pakan et al.
The Journal of comparative neurology, 501(4), 619-630 (2007-02-06)
Purkinje cells in the cerebellum express the antigen zebrin II (aldolase C) in many vertebrates. In mammals, zebrin is expressed in a parasagittal fashion, with alternating immunopositive and immunonegative stripes. Whether a similar pattern is expressed in birds is unknown.
Subpopulations of neurons expressing parvalbumin in the human amygdala
Pantazopoulos H, et al.
The Journal of Comparative Neurology, 496(5), 706-722 (2006)
beta-Catenin is critical for cerebellar foliation and lamination
Wen J, et al.
Testing, 8(5), e64451-e64451 (2013)
Altered hippocampal expression of calbindin-D-28k and calretinin in GABAB (1)-deficient mice
Ruttimann E, et al.
Biochemical Pharmacology, 68(8), 1613-1620 (2004)
A I Soto-Ortolaza et al.
European journal of neurology, 17(2), 208-211 (2009-08-14)
Calcium levels have been proposed to play an important role in the selective vulnerability of nigrostriatal dopaminergic neurons in Parkinson's disease (PD). Recently, an association was reported between the calcium buffer, calbindin (rs1805874) and risk of PD in a Japanese
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