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C6198

抗-肌动蛋白, α-平滑肌- Cy3™抗体，小鼠单克隆

clone 1A4, purified from hybridoma cell culture

别名:

单克隆抗-肌动蛋白，α-平滑肌, α-平滑肌肌动蛋白- Cy3™抗体, α-肌动蛋白2 Cy3, SMA

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关于此项目

NACRES:
NA.43
UNSPSC Code:
12352203
Conjugate:
CY3 conjugate
Clone:
1A4, monoclonal
Application:
IHC (p)
Citations:
897
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biological source

mouse

Quality Level

200

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1A4, monoclonal

form

buffered aqueous solution

mol wt

antigen ~42 kDa

species reactivity

human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake

storage condition

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tonsil or appendix

isotype

IgG2a

UniProt accession no.

P62736

application(s)

research pathology

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)

General description

其抗体（也称为抗α-Sm-1）对α-平滑肌肌动蛋白的单一异构体具有特异性。它在免疫印迹试验中可与α-平滑肌肌动蛋白发生特异性反应，并可在冷冻或福尔马林固定的、或石蜡包埋的组织切片中对平滑肌细胞进行标记。
单克隆抗肌动蛋白α-平滑肌（小鼠IgG2a同种型）源自小鼠骨髓瘤细胞和免疫BALB/c小鼠脾细胞融合产生的1A4杂交瘤

Immunogen

N-末端合成的α-平滑肌肌动蛋白十肽。

Application

使用单克隆抗ACTA2抗体对来自牛主动脉的平滑肌细胞进行免疫细胞化学分析。首先将细胞在玻璃盖玻片上生长，并在4％的50％丙酮/乙醇中固定10分钟。
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
这一抗体成功运用案例，以及相关综述文献，如下：
流式细胞仪/细胞分选（2篇文献）
免疫组织化学（3篇）
免疫荧光（13篇）
免疫组织化学（27篇）
Western印迹法（2篇）

Biochem/physiol Actions

在鸡和大鼠胚胎成肌过程中，平滑肌肌动蛋白可与肌节α-肌动蛋白进行瞬时共表达。在成年哺乳动物心脏的心室传导通道中，经证存在这种物质。它在平滑肌瘤、平滑肌肉瘤和平滑肌母细胞瘤以及部分横纹肌肉瘤中表达。

Physical form

溶解保存在0.01M磷酸缓冲盐,pH7.4, 含有1%牛血清白蛋白和15 mM叠氮化钠。

Other Notes

想要查看肌动蛋白抗体选择指南，请访问 www.sigmaaldrich.com/actin。

Legal Information

Cy在 Amersham Biosciences Limited许可下销售。
Cy3 is a trademark of Cytiva

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

低风险生物材料
常规特殊物品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000476898
0000476897
0000433479
0000433479
0000439655

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Data Sheet - C6198

Instructions

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全球贸易项目编号

货号GTIN
C6198-.2ML04061833510964
C6198-100UL04061833510971