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C5914

Sigma-Aldrich

非酶细胞裂解液 1x

Prepared in phosphate buffered saline without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture

别名:

Dissociation Solution

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About This Item

MDL编号:
MFCD00282844
UNSPSC代码:
12352204
NACRES:
NA.75

质量水平

200

无菌性

sterile-filtered

产品线

BioReagent

形式

liquid

浓度

1 ×

技术

cell culture | mammalian: suitable

pH值（酸碱度）

6.9-7.1

运输

wet ice

储存温度

2-8°C

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相关类别

一般描述

在人间充质细胞中通过非酶方法进行的细胞分离有助于脱离并保持其功能，特别是细胞表面趋化因子受体的表达。

应用

细胞解离液非酶1x已用于视网膜星形胶质细胞培养基、 U87胶质母细胞瘤肿瘤细胞和心肌干细胞(CSC)的解离。
特殊的非酶配方，用于从塑料或玻璃表面温和地剥离贴壁细胞。

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Can one use Product C5914, Cell Dissociation Solution, with tissue?

    We have only used with monolayer cell culture, and therefore we have no information on its usage with tissue.

  6. Does Product C5914, Cell Dissociation Solution, contain EDTA?

    Yes, it does contain EDTA; the concentration is proprietary.

  7. Does Product C5914, Cell Dissociation Solution, work with epithelial cells?

    It will work with most cell types.

  8. When trypsin is used to detach cells, one usually adds fetal bovine serum (FBS) to the media. Should FBS be used with Product C5914, Cell Dissociation Solution?

    No. FBS is used to inactivate trypsin. This product is not enzymatic, so there is no need to use FBS. The cells can be washed with phosphate buffered saline (PBS). When the cells are to be cultured, then one would need to add FBS.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Journal of Nuclear Medicine, 50(7), 1124-1130 (2009)
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Matsuda T, et al.
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