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表单
lyophilized powder
质量水平
比活
≥10 units/mg solid
分子量
~95 kDa
技术
cell based assay: suitable
适用性
suitable for assay of choline
suitable for molecular biology
储存温度
−20°C
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应用
来自产碱杆菌属的胆碱氧化酶已用于：
- 在一项研究中研究用几丁质膜构建的胆碱生物传感器及其在测定胆碱酯酶抑制活性中的应用
- 胆碱氧化酶与共价结合黄素的光反应的光谱研究。
- 作为磷酸盐缓冲液 (PBS) 缓冲液的成分，用于测定脑组织匀浆中的胆碱乙酰转移酶 (ChAT) 活性
- 通过 ATX 活性试验测定自分泌运动因子 (ATX) 产生的胆碱，通过 PLD 活性试验测定磷脂酶-D (PLD) 产生的胆碱
生化/生理作用
胆碱氧化酶可利用甜菜碱醛作为中间体而分子氧作为次要电子受体，催化胆碱至甘氨酸-甜菜碱的四电子氧化。该酶还可接受甜菜碱醛作为底物。这使得对将胆碱转化为醛中间体、＆amp; 甜菜碱醛转化为甘氨酸-甜菜碱的反应机制研究成为可能。该酶是一种分子量约为72,000 Da（凝胶过滤）或66,000 Da（SDS凝胶电泳）的黄酮蛋白。
物理属性
等电点：4.1 ± 0.1
米氏常数：2.84 x 10-3M (胆碱), 5.33 x 10¯3M (甜菜醛)
结构：一摩尔的FAD与一摩尔的酶共价结合
抑制剂：对氯苯甲酸，Cu++，Co++，Hg++，Ag+
最适pH：8.0 – 8.5
最适温度：40 – 45°C
pH稳定性： pH 7.0 – 9.0 (30°C, 2小时)
热稳定性： 低于37°C (pH 7.5, 10分钟)
米氏常数：2.84 x 10-3M (胆碱), 5.33 x 10¯3M (甜菜醛)
结构：一摩尔的FAD与一摩尔的酶共价结合
抑制剂：对氯苯甲酸，Cu++，Co++，Hg++，Ag+
最适pH：8.0 – 8.5
最适温度：40 – 45°C
pH稳定性： pH 7.0 – 9.0 (30°C, 2小时)
热稳定性： 低于37°C (pH 7.5, 10分钟)
单位定义
一单位的酶在37°C、pH 8.0条件下每分钟内可将1 μ摩尔的胆碱氧化成甜菜碱，并同时生成1 μ摩尔的H2O2。注：在通过胆碱氧化酶进行胆碱向甜菜碱的转化过程中，每μ摩尔的胆碱将产生2μ摩尔的H2O2。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
M Ohta-Fukuyama et al.
Journal of biochemistry, 88(1), 197-203 (1980-07-01)
Choline oxidase from Alcaligenes sp. catalyzed the oxidation of choline and betaine aldehyde to betaine with concomitant consumption of oxygen and production of hydrogen peroxide. The values of Km for choline and betaine aldehyde were 0.87 and 6.2 mM, respectively.
Bo-Chuan Hsieh et al.
Journal of pharmaceutical and biomedical analysis, 45(4), 673-678 (2007-03-03)
An amperometric flow-injection choline biosensor was assembled utilizing natural chitinous membrane as the supporting material for biocatalyst immobilization, and the membrane was purified from Taiwanese soldier crab, Mictyris brevidactylus. The chitinous membrane (<50.0 microm in thickness) was covalently immobilized with
Rungaroon Waditee et al.
The Journal of biological chemistry, 278(7), 4932-4942 (2002-12-06)
Glycine betaine (N,N,N-trimethylglycine) is an important osmoprotectant and is synthesized in response to abiotic stresses. Although almost all known biosynthetic pathways of betaine are two-step oxidation of choline, here we isolated two N-methyltransferase genes from a halotolerant cyanobacterium Aphanothece halophytica.
Samantha R Eck et al.
Biological psychiatry, 88(7), 566-575 (2020-07-01)
Stress exacerbates symptoms of schizophrenia and attention-deficit/hyperactivity disorder, which are characterized by impairments in sustained attention. Yet how stress regulates attention remains largely unexplored. We investigated whether a 6-day variable stressor altered sustained attention and the cholinergic attention system in
M C Carou et al.
Acta histochemica, 119(5), 462-470 (2017-05-17)
Follicular atresia in granulosa and theca cells occurs by apoptosis through weak hormonal stimulation. We have previously proposed an in vitro model to study this process by inducing apoptosis in BGC-1, a bovine granulosa cell line, and in primary cultures
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