C5896
胆碱氧化酶 来源于产碱杆菌 属
lyophilized powder, ≥10 units/mg solid
别名:
胆碱：氧气 1-氧化还原酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-840-0
MDL number:
Specific activity:
≥10 units/mg solid
form
lyophilized powder
specific activity
≥10 units/mg solid
mol wt
~95 kDa
technique(s)
cell based assay: suitable
suitability
suitable for assay of choline, suitable for molecular biology
storage temp.
−20°C
Quality Level
General description
等电点：4.1 ± 0.1
米氏常数：2.84 x 10-3M (胆碱), 5.33 x 10¯3M (甜菜醛)
结构：一摩尔的FAD与一摩尔的酶共价结合
抑制剂：对氯苯甲酸，Cu++，Co++，Hg++，Ag+
最适pH：8.0 – 8.5
最适温度：40 – 45°C
pH稳定性： pH 7.0 – 9.0 (30°C, 2小时)
热稳定性： 低于37°C (pH 7.5, 10分钟)
米氏常数：2.84 x 10-3M (胆碱), 5.33 x 10¯3M (甜菜醛)
结构：一摩尔的FAD与一摩尔的酶共价结合
抑制剂：对氯苯甲酸，Cu++，Co++，Hg++，Ag+
最适pH：8.0 – 8.5
最适温度：40 – 45°C
pH稳定性： pH 7.0 – 9.0 (30°C, 2小时)
热稳定性： 低于37°C (pH 7.5, 10分钟)
Application
来自产碱杆菌属的胆碱氧化酶已用于：
- 在一项研究中研究用几丁质膜构建的胆碱生物传感器及其在测定胆碱酯酶抑制活性中的应用
- 胆碱氧化酶与共价结合黄素的光反应的光谱研究。
- 作为磷酸盐缓冲液 (PBS) 缓冲液的成分，用于测定脑组织匀浆中的胆碱乙酰转移酶 (ChAT) 活性
- 通过 ATX 活性试验测定自分泌运动因子 (ATX) 产生的胆碱，通过 PLD 活性试验测定磷脂酶-D (PLD) 产生的胆碱
Biochem/physiol Actions
胆碱氧化酶可利用甜菜碱醛作为中间体而分子氧作为次要电子受体，催化胆碱至甘氨酸-甜菜碱的四电子氧化。该酶还可接受甜菜碱醛作为底物。这使得对将胆碱转化为醛中间体、＆amp; 甜菜碱醛转化为甘氨酸-甜菜碱的反应机制研究成为可能。该酶是一种分子量约为72,000 Da（凝胶过滤）或66,000 Da（SDS凝胶电泳）的黄酮蛋白。
Other Notes
一单位的酶在37°C、pH 8.0条件下每分钟内可将1 μ摩尔的胆碱氧化成甜菜碱，并同时生成1 μ摩尔的H2O2。注：在通过胆碱氧化酶进行胆碱向甜菜碱的转化过程中，每μ摩尔的胆碱将产生2μ摩尔的H2O2。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Bo-Chuan Hsieh et al.
Journal of pharmaceutical and biomedical analysis, 45(4), 673-678 (2007-03-03)
An amperometric flow-injection choline biosensor was assembled utilizing natural chitinous membrane as the supporting material for biocatalyst immobilization, and the membrane was purified from Taiwanese soldier crab, Mictyris brevidactylus. The chitinous membrane (<50.0 microm in thickness) was covalently immobilized with
M Ohta-Fukuyama et al.
Journal of biochemistry, 88(1), 197-203 (1980-07-01)
Choline oxidase from Alcaligenes sp. catalyzed the oxidation of choline and betaine aldehyde to betaine with concomitant consumption of oxygen and production of hydrogen peroxide. The values of Km for choline and betaine aldehyde were 0.87 and 6.2 mM, respectively.
Giovanni Gadda
Biochimica et biophysica acta, 1646(1-2), 112-118 (2003-03-15)
Choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as intermediate and molecular oxygen as primary electron acceptor. The enzyme is capable of accepting betaine-aldehyde as a substrate, allowing the investigation of the reaction mechanism for both
Shengyuan Deng et al.
Biosensors & bioelectronics, 26(11), 4552-4558 (2011-06-10)
A signal amplification system for electrochemiluminescence (ECL) of quantum dots (QDs) was developed by using electrochemically reduced graphene oxide (ERGO) to construct a nanobiosensing platform. Due to the structural defects of GO, the ECL emission of QDs coated on GO
S Pundir et al.
Analytical biochemistry, 427(1), 26-32 (2012-05-09)
A bienzymatic choline biosensor was constructed by coimmobilizing acetylcholinesterase (AChE) and choline oxidase (ChO) onto nanocomposite of carboxylated multiwalled carbon nanotubes (c-MWCNTs) and zirconium oxide nanoparticles (ZrO(2)NPs) electrodeposited on the surface of a glassy carbon electrode (GCE) and using it
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