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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
LhS 28, monoclonal
表单
buffered aqueous solution
分子量
antigen ~45 kDa
种属反应性
human, hamster
浓度
~2 mg/mL
技术
electron microscopy: suitable
immunocytochemistry: 1-2 μg/mL using BHK a21 cells
immunohistochemistry: suitable
microarray: suitable
western blot: suitable
同位素/亚型
IgG1
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
Anti-Ciliated Cell Marker antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma LhS 28 produced by the fusion of mouse myeloma cells (Sp2/0 cells) and splenocytes from BALB/c mice.
特异性
Anti-Ciliated Cell Marker antibody, Mouse monoclonal recognizes an antigen in human and hamster ciliated cells.
免疫原
BHK a21 cytoskeletal preparation.
应用
Anti-Ciliated Cell Marker antibody, Mouse monoclonal may be used in:
- immunoelectron microscopy
- immunoblotting
- immunohistochemistry
- immunocytochemistry
May be used in the detection of ciliated cells and for investigating mechanisms of ciliogenesis.
生化/生理作用
The mouse hybridoma LhS 28 secretes a monoclonal antibody that recognizes the antigen 44-45 kDa protein associated with the ciliary basal bodies. The antigen recognized by LhS 28 is present in the 9+0 microtubule arrays in the basal body structure of the cilium. It serves as an unequivocal marker for in situ detection and in vitro studies of human ciliated epithelial cells. LhS28 is a potential marker for tubal metaplasia identification and may be useful in recognizing low grade cervical glandular intraepithelial neoplasia. It is effective in detecting ciliogenesis and ciliated cells associated with normal and pathological human specimens
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
储存及稳定性
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
M T Comer et al.
Journal of clinical pathology, 52(5), 355-357 (1999-11-24)
The assessment of neoplastic disease in gynaecological histopathology can be complicated by the high incidence of metaplasia seen in tissues of the female genital tract. There is a need to identify specific tissue markers which can be applied in routine
M T Comer et al.
The Histochemical journal, 31(1), 39-43 (1999-07-16)
The process and regulation of ciliogenesis in human epithelia is little understood and many components of the cilium and associated structures have not been characterised. We have identified a monoclonal antibody, LhS28, which recognises a 44,000-45,000 M(r) protein specifically associated
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