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Merck
CN

C5838

Sigma-Aldrich

单克隆抗肌动蛋白抗体

clone AC-40, purified immunoglobulin, buffered aqueous solution

别名:

Monoclonal Anti-Actin

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

mouse

质量水平

偶联物

CY3 conjugate

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

AC-40, monoclonal

形式

buffered aqueous solution

分子量

antigen 42 kDa

种属反应性

human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat

技术

direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts

同位素/亚型

IgG2a

运输

wet ice

储存温度

2-8°C

靶向翻译后修饰

unmodified

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相关类别

一般描述

该抗体可识别在所有肌动蛋白亚型中均保守的C末端表位。它能够特异性标记各种组织和物种中的肌动蛋白。 被抗体识别的表位能够耐受甲醛固定和石蜡包埋。锌-福尔马林、B5、乙醇、methacarn、Brunnel's或Bouin's溶液也可用作固定液。
Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin has six isoforms. Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. There are three α-actins (α-skeletal, α-cardiac and α-smooth muscle), one β-actin (β-nonmuscle) and two γ-actins (γ-smooth muscle and γ-nonmuscle).

免疫原

与多抗原肽(MAP)骨架连接的合成肌动蛋白C末端肽

应用

已使用肌动蛋白单克隆抗体作为一抗,对MDCK细胞裂解物进行蛋白质免疫印迹分析。
肌动蛋白单克隆抗体被用于Cos-7细胞裂解物的蛋白质免疫印迹分析,以确保等量蛋白质上样。
Monoclonal Anti-Actin-Cy3 antibody has been used in immunofluorescent staining.

生化/生理作用

Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

其他说明

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

法律信息

Distributed under license from Amersham Biosciences Limited.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Erin Faught et al.
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The mineralocorticoid receptor (MR) in mammals mediates the effects of aldosterone in regulating fluid balance and potassium homeostasis. While MR signalling is essential for survival in mammals, there is no evidence that MR has any physiological role in ray-finned fish.
Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.
Gunning P, et al.
Molecular and Cellular Biology, 3(5), 787-795 (1983)
Wentao Shi et al.
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Ectomesenchymal stem cells (EMSCs) represent a type of adult stem cells derived from the cranial neural crest. These cells are capable of self‑renewal and have the potential for multidirectional differentiation. Tissue transglutaminase type 2 (TG2) is a ubiquitously expressed member
A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation.
Skalli O, et al.
The Journal of Cell Biology, 103(6), 2787-2796 (1986)
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Sialic acids are important components of glycoproteins and glycolipids essential for cellular communication, infection, and metastasis. The importance of sialic acid biosynthesis in human physiology is well illustrated by the severe metabolic disorders in this pathway. However, the biological role

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