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一般描述
细胞非酶解离溶液缓冲系统可替代用于粘附间充质干细胞(MSC)单层解离的基于标准胰蛋白酶的缓冲液。
应用
细胞非酶解离溶液1X已用于鼠巨噬细胞(RAW2647)细胞、A549肺细胞、胰岛细胞以及2D细胞和3D乳腺球群细胞的解离。
特殊的非酶配方,用于从塑料或玻璃表面温和地剥离贴壁细胞。
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
Differential effects of RU486 reveal distinct mechanisms for glucocorticoid repression of prostaglandin E2 release
European Journal of Biochemistry, 271(20), 4042-4052 (2004)
Comparison of enzymatic and non-enzymatic means of dissociating adherent monolayers of mesenchymal stem cells
Biological Procedures Online, 11(1), 161-161 (2009)
Impact of integrin-matrix matching and inhibition of apoptosis on the survival of purified human beta-cells in vitro
Diabetologia, 45(6), 841-850 (2002)
Evidence for lipopolysaccharide induced differentiation of RAW264. 7 murine macrophage cell line into dendritic like cells
Journal of Biosciences, 28(1), 129-134 (2003)
Frontiers in immunology, 11, 1643-1643 (2020-08-28)
Introduction: Proteinuria contributes to progression of renal damage, partly by complement activation on proximal tubular epithelial cells. By pattern recognition, properdin has shown to bind to heparan sulfate proteoglycans on tubular epithelium and can initiate the alternative complement pathway (AP).
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