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Merck
CN
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文件

C4951

Sigma-Aldrich

胆固醇 - 水溶性

powder, BioReagent, suitable for cell culture

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别名:
胆固醇 - 甲基-β-环糊精
UNSPSC代码:
12352211
NACRES:
NA.75

生物来源

ovine wool

质量水平

200

产品线

BioReagent

形式

powder

技术

cell culture | mammalian: suitable

溶解性

H2O: 300 mg/mL

运输

ambient

储存温度

−20°C

相关类别

应用

水溶性胆固醇可用于：
  • 大鼠平滑肌细胞(SMC)游离胆固醇(FC)过载
  • 细胞毒性试验测定
  • 培养感染幽门螺杆菌的响应CD4+ T细胞的培养
  • 研究SH-SY5Y人神经母细胞瘤细胞膜胆固醇水平
  • 研究胆固醇对原代培养大鼠海马神经元中细胞活性和3-(4,5-二甲基噻唑-2-乙基)-2,5-二苯基溴化四唑(MTT)还原的影响
  • 作为用作细菌培养生长的补充剂
  • 检查S蛋白与血管紧张素转换酶2(ACE2)的相互作用
  • 研究胆固醇对豚鼠内毛细胞钾电流的影响
  • 可逆性抑制对蛋白酶K的可逆性抑制可逆性抑制

包装

包装规格取决于胆固醇

其他说明

每克含有约40 mg胆固醇；平衡甲基-β-环糊精。

制备说明

配制 300 mg/mL 的水溶液。 稀释后使溶液静置一小时，以使产品完全溶解。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How much cholesterol will I be getting in the 30 mg package of Product C4951, Cholesterol-Water Soluble?

    The package size is based on the amount of cholesterol present.  There is approximately 40 mg of cholesterol per gram of material, the balance being Methyl-β-cyclodextrin.

  4. What is the molecular weight of Product C4951, Cholesterol-Water Soluble?

    386.65 g/mol

  5. How do I calculate the molarity of Product C4951, Cholesterol-Water Soluble?

    Calculate the molarity based on the cholesterol content of your solution.  For instance, if you dissolve 10 mg of material in water, approximately 0.4 mg of that 10 mg is cholesterol.

  6. What concentration of Product C4951, Cholesterol-Water Soluble, is used in the cell culture test?

    The water soluble cholesterol is tested for cell culture use at approximately 7.6 micrograms per/L.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Barbora Melkes et al.
Naunyn-Schmiedeberg's archives of pharmacology, 389(12), 1289-1300 (2016-09-08)
There are some indications that biased μ-opioid ligands may diversely affect μ-opioid receptor (MOR) properties. Here, we used confocal fluorescence recovery after photobleaching (FRAP) to study the regulation by different MOR agonists of receptor movement within the plasma membrane of
Cholesterol influences potassium currents in inner hair cells isolated from guinea pig cochlea
Kimitsuki T
Auris, Nasus, Larynx, 44(1), 46-51 (2017)
Autophagy plays a protective role in free cholesterol overloading induced smooth muscle cells death
Xu K, et al.
Journal of Lipid Research, jlr-M005702 (2010)
Cholesterol does not affect the toxicity of amyloid beta fragment but mimics its effect on MTT formazan exocytosis in cultured rat hippocampal neurons
Abe K and Saito H
Neuroscience Research, 35(3), 165-174 (1999)
A novel reciprocal and biphasic relationship between membrane cholesterol and beta-secretase activity in SH-SY5Y cells and in human platelets
Liu WW, et al.
Journal of Neurochemistry, 108(2), 341-349 (2009)
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