C4874
钙调蛋白 牛
recombinant, expressed in E. coli, lyophilized powder, ≥98% (SDS-PAGE)
别名:
磷酸二酯酶3:5-环核苷酸激活剂, 磷酸二酯酶 3′:5′-环核苷酸活化剂
产品名称
钙调蛋白 牛, recombinant, expressed in E. coli, lyophilized powder, ≥98% (SDS-PAGE)
biological source
bovine
recombinant
expressed in E. coli
assay
≥98% (SDS-PAGE)
form
lyophilized powder
mol wt
Mw 19000.9 by amino acid sequence
composition
Protein, ≥85%
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
bovine ... CALM(100297344)
Application
使用牛钙调蛋白对重组维甲酸诱导基因I蛋白进行体外磷酸化分析。此外，它还用于内源性蛋白酶Glu-C蛋白水解和脱酰胺研究。
Biochem/physiol Actions
激活环核苷酸依赖性磷酸二酯酶所需的Ca2+结合蛋白。它也是一氧化氮合酶、钙调神经磷酸酶和许多激酶的辅因子/激活剂，包括ATP酶、肌球蛋白轻链激酶和蛋白激酶I、II和III。它通过环ADP核糖介导兰尼碱受体激活，并参与细胞内Ca2+稳态。
牛钙调蛋白在钙结合时发生构型变化。它与鞘氨酰磷酰胆碱结合，抑制钙调磷酸酶和磷酸二酯酶。
General description
序列：
MGSSHHHHHHSSGLVPRGSHMADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
MGSSHHHHHHSSGLVPRGSHMADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
牛钙调蛋白具有哑铃式结构。两个钙结合EF手环、反平行β片和三个α螺旋线组成一个叶。它有一个与叶相连的中心螺旋线。钙调蛋白能以协同交互方式结合四个钙分子。
Preparation Note
使用没有动物组分的材料生产。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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