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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
hHCD, monoclonal
包含
15 mM sodium azide
种属反应性
sheep, rabbit, human, pig, bovine, mouse
技术
immunohistochemistry: 1:500 using methacarn-fixed, paraffin-embedded sections of human or animal tissue
immunoprecipitation (IP): suitable
western blot: 1:4,000 using human uterus extract
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... CALD1(800)
mouse ... Cald1(109624)
特异性
The antibody (also cited as h-CD) reacts in immunoblotting assays with caldesmon polypeptide of 150 kDa (h-caldesmon). It does not cross-react with skeletal or cardiac muscle or with the 70 kDa non-muscle caldesmon. In immunohistochemical staining, the antibody exhibits smooth muscle specificity. It stains vascular and visceral smooth muscle cells but not epithelial, endothelial or connective tissue fibroblast cells. In normal and malignant breast tissue, the myoepithelial component of galactophorous sinuses (but not in ducts of lobules) is stained; however, the antibody may be non-reactive by immunocytochemical methods with cultured smooth muscle cells. This probably reflects the down regulation of caldesmon commonly observed in tissue culture.
免疫原
human uterus smooth muscle extract.
应用
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-Caldesmon (Smooth) antibody produced in mouse is suitable for:
- immunohistochemistry at a dilution of 1:500 using methacarn-fixed, paraffin-embedded sections of human or animal tissue
- immunoprecipitation
- western blot at a dilution of 1:4,000 using human uterus extract
生化/生理作用
Monoclonal Anti-Caldesmon (Smooth) (mouse IgG1 isotype) reacts in immunoblotting assays with caldesmon polypeptide of 150 kDa (h-Caldesmon).
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
PloS one, 8(2), e57451-e57451 (2013-02-26)
Adequate blood flow through placental chorionic plate resistance arteries (CPAs) is necessary for oxygen and nutrient transfer to the fetus and a successful pregnancy. In non-placental vascular smooth muscle cells (SMCs), K(+) channels regulate contraction, vascular tone and blood flow.
Biomaterials, 206, 41-48 (2019-03-30)
Stress urinary incontinence (SUI) is a life changing condition, affecting 20 million women worldwide. In this study, we developed a bioactive, injectable bulking agent that consists of Permacol™ (Medtronic, Switzerland) and recombinant insulin like growth factor-1 conjugated fibrin micro-beads (fib_rIGF-1)
Developmental biology, 153(2), 185-193 (1992-10-01)
Expression of the regulatory contractile proteins, heavy caldesmon (h-caldesmon) and calponin was studied in human aortic smooth muscle cells (SMCs) during development and compared with the expression of alpha-SM-actin and smooth muscle-myosin heavy chain (SM-MHCs). For this study, novel monoclonal
Isolation, culture, and characterization of human intestinal smooth muscle cells.
Methods in molecular medicine, 78, 417-423 (2003-06-27)
Journal of vascular research, 45(5), 365-374 (2008-03-21)
The success of peripheral vein grafts is limited by intimal hyperplasia. Transforming growth factor (TGF)-beta(1) has effects on cell proliferation, apoptosis and extracellular matrix synthesis. We have previously observed positive changes in vessel healing with antisense to TGF-beta(1). Adenovirus was
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