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Merck
CN

C3956

Sigma-Aldrich

抗-c-Myc 兔抗

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

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别名:
Myc Tag Polyclonal Antibody, c-Myc Antibody
MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.56

生物来源

rabbit

质量水平

偶联物

unconjugated

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

形式

buffered aqueous solution

种属反应性

human

浓度

~0.5 mg/mL

技术

immunocytochemistry: 5-10 μg/mL
immunoprecipitation (IP): 1-2 μg using immunoprecipitates a c-Myc fusion protein from transfected mammalian cell lysates or bacterial extracts
indirect immunofluorescence: 5-10 μg/mL using detects c-Myc fusion proteins in methanol-acetone fixed transiently transfected cells
microarray: suitable
western blot: 0.5-1 μg/mL using detects c-Myc fusion proteins in cell extracts from transfected cultures as well as bacterial lysates.

UniProt登记号

储存温度

−20°C

基因信息

human ... MYC(4609)

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一般描述

MYC(MYC原致肿瘤基因)是一种转录因子和癌蛋白。它位于染色体8q24。c-Myc基因编码碱性螺旋-环-螺旋/亮氨酸拉链(bHLH/LZ)转录因子。
抗-c-Myc抗体用于检测c-Myc标记融合蛋白的c-Myc标记序列。这一多克隆抗体在兔子体内产生,识别在N末端和C末端的c-Myc序列。C3956是一种亲和纯化抗体,在大多数应用中增强灵敏度。

特异性

c-Myc (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu)标记融合蛋白N末端或C末端。

免疫原

人c-myc原致肿瘤基因的肽链对应氨基酸408-425,通过C-末端加成半胱氨酸残基和马来酰亚胺-激活KLH偶联。

应用

抗-c-Myc抗体可以用于western印迹分析 和化学发光检测。
抗体可以用于免疫印迹,免疫沉淀和免疫荧光。

生化/生理作用

MYC(MYC原致肿瘤基因)对于癌症起始,发展和对治疗抵抗性是很重要的。DLBCL(弥漫性大B细胞淋巴瘤)中MYC过表达导致使用利妥昔单抗联合环磷酰胺,阿霉素,长春新碱和强的松(R-CHOP)进行侵入性治疗时效果很差。细胞髓细胞组织增生(c-myc)肿瘤基因在细胞增生,分化和凋亡中有重要作用。它可用作基因表达的转录调控因子。

外形

溶于0.01M磷酸盐缓冲盐水,pH 7.4,含有1%牛血清白蛋白以及15 mM叠氮化钠。

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品

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