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Merck
CN

C3956

抗-c-Myc 兔抗

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

别名:

Myc Tag Polyclonal Antibody, c-Myc Antibody

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.56
MDL number:
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产品名称

抗-c-Myc 兔抗, ~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

concentration

~0.5 mg/mL

technique(s)

immunocytochemistry: 5-10 μg/mL
immunoprecipitation (IP): 1-2 μg using immunoprecipitates a c-Myc fusion protein from transfected mammalian cell lysates or bacterial extracts
indirect immunofluorescence: 5-10 μg/mL using detects c-Myc fusion proteins in methanol-acetone fixed transiently transfected cells
microarray: suitable
western blot: 0.5-1 μg/mL using detects c-Myc fusion proteins in cell extracts from transfected cultures as well as bacterial lysates.

UniProt accession no.

storage temp.

−20°C

Quality Level

Gene Information

human ... MYC(4609)

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Physical form

溶于0.01M磷酸盐缓冲盐水,pH 7.4,含有1%牛血清白蛋白以及15 mM叠氮化钠。

Application

抗-c-Myc抗体可以用于western印迹分析 和化学发光检测。
抗体可以用于免疫印迹,免疫沉淀和免疫荧光。

Biochem/physiol Actions

MYC(MYC原致肿瘤基因)对于癌症起始,发展和对治疗抵抗性是很重要的。DLBCL(弥漫性大B细胞淋巴瘤)中MYC过表达导致使用利妥昔单抗联合环磷酰胺,阿霉素,长春新碱和强的松(R-CHOP)进行侵入性治疗时效果很差。细胞髓细胞组织增生(c-myc)肿瘤基因在细胞增生,分化和凋亡中有重要作用。它可用作基因表达的转录调控因子。
c-Myc (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu)标记融合蛋白N末端或C末端。

General description

MYC(MYC原致肿瘤基因)是一种转录因子和癌蛋白。它位于染色体8q24。c-Myc基因编码碱性螺旋-环-螺旋/亮氨酸拉链(bHLH/LZ)转录因子。
抗-c-Myc抗体用于检测c-Myc标记融合蛋白的c-Myc标记序列。这一多克隆抗体在兔子体内产生,识别在N末端和C末端的c-Myc序列。C3956是一种亲和纯化抗体,在大多数应用中增强灵敏度。

Immunogen

人c-myc原致肿瘤基因的肽链对应氨基酸408-425,通过C-末端加成半胱氨酸残基和马来酰亚胺-激活KLH偶联。

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存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Sarkar S, et al.
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Infection and immunity, 75(3), 1116-1128 (2006-12-13)
Macrophage migration inhibitory factor (MIF) is a mammalian cytokine that participates in innate and adaptive immune responses. Homologues of mammalian MIF have been discovered in parasite species infecting mammalian hosts (nematodes and malaria parasites), which suggests that the parasites express
The role of low-density receptor-related protein 1 (LRP1) as a competitive substrate of the amyloid precursor protein (APP) for BACE1.
von Einem B, et al.
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Ribosomal proteins RPL37, RPS15 and RPS20 regulate the Mdm2-p53-MdmX network.
Daftuar L, et al.
PLoS ONE, 8(7), e68667-e68667 (2013)
FDG-PET is a good biomarker of both early response and acquired resistance in BRAFV600 mutant melanomas treated with vemurafenib and the MEK inhibitor GDC-0973.
Baudy AR, et al.
EJNMMI Research, 2(1), 22-22 (2012)

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