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安全信息

C3934

Sigma-Aldrich

碳酸酐酶 来源于牛红细胞

lyophilized powder, ≥2,000 W-A units/mg protein

别名:

碳酸盐脱水酶, 碳酸酯水解酶

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About This Item

CAS号:
9001-03-0
EC 号:
4.2.1.1 (BRENDA, IUBMB)
EC 号:
232-576-6
MDL编号:
MFCD00081467
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

bovine erythrocytes

质量水平

300

形式

lyophilized powder

比活

≥2,000 W-A units/mg protein

分子量

30 kDa

应用

diagnostic assay manufacturing

储存温度

2-8°C

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应用

牛红血球(BCA)来源碳酸酐酶已用于研究去除酶结合金属离子Zn2+对酶的聚集行为的影响。使用螯合剂（例如EDTA（乙二胺四乙酸） ）去除金属离子，增强了酶采用熔融球状态的倾向。人们发现，这种状态的酶可与分子伴侣样α-晶状体蛋白结合并防止聚集。来自Sigma的酶已固定在电化学传感器上，以便开发出彻底溶解CO2的分析装置。其还用于BCA构象变化的热力学分析。

生化/生理作用

碳酸酐酶是一种锌金属酶，其分子量约为30,000Da。该酶催化二氧化碳水合成碳酸。它参与重要的生理和病理过程，如pH和CO2稳态、碳酸氢盐和CO2的转运、生物合成反应、骨吸收、钙化和致肿瘤性。因此，该酶是用于各种病理学（如青光眼、癫痫和帕金森病）的临床应用的抑制剂的重要靶标。

单位定义

在0°C条件下，一Wilbur-Anderson (W-A)单位可使0.02M Trizma缓冲液pH在一分钟内从8.3降至 6.3。（一W-A单位基本上相当于一Roughton-Booth单位。）

抑制剂

产品编号
说明
价格

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the molecular weight of Product C3934, Carbonate Dehydratase?

    Because there are a number of closely related isoforms, the molecular weight typically used is approximately 30 kDa.  This product is expected to be a mixture of isoforms.

  4. What is the purity of Product C3934, Carbonate Dehydratase?

    Since C3934 is a mixture of isoforms, no purity claim is made.  The product is packaged and offered based on its enzymatic activity.

  5. Can stock solutions of Product C3934, Carbonate Dehydratase, be made and stored?

    Carbonic anhydrase solutions can be prepared in water or buffer at concentrations up to 10 mg/mL.  It is common for there to be some particulates or haziness in the solution.  No data on solution stability has been collected by Sigma-Aldrich, so we recommend solutions be prepared fresh for use.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Use of carbonic anhydrase in electrochemical biosensors for dissolved CO2
Cammaroto C, et al.
Sensors and Actuators B, Chemical, 48(1-3), 439-447 (1998)
Fraser J Moss et al.
The Journal of physiology, 598(24), 5821-5856 (2020-09-25)
According to the HCO 3 - metabolon hypothesis, direct association of cytosolic carbonic anhydrases (CAs) with the electrogenic Na/HCO3 cotransporter NBCe1-A speeds transport by regenerating/consuming HCO 3 - . The present work addresses published discrepancies as to whether
Mikhail Krasavin et al.
Journal of enzyme inhibition and medicinal chemistry, 35(1), 165-171 (2019-11-23)
Testing of an expanded, 800-compound set of analogues of the earlier described Strecker-type α-aminonitriles (selected from publicly available Enamine Ltd. Screening Collection) in thermal shift assay against bovine carbonic anhydrase (bCA) led to further validation of this new class of
A carbonic anhydrase gene is induced in the nodule primordium and its cell-specific expression is controlled by the presence of Rhizobium during development.
Coba de la Pe??a, et al.
The Plant Journal, 11, 407-420 (2019)
Mikhail Krasavin et al.
Journal of enzyme inhibition and medicinal chemistry, 35(1), 306-310 (2019-12-05)
The differential scanning fluorimetry (DSF) screening of 5.692 fragments in combination with benzenesulfonamide (BSA) against bovine carbonic anhydrase (bCA) delivered >100 hits that either caused, on their own, a significant thermal shift (ΔTm, °C) in the protein melting temperature or
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实验方案

Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)

Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)

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