C3934
碳酸酐酶 来源于牛红细胞
lyophilized powder, ≥2,000 W-A units/mg protein
别名:
碳酸盐脱水酶, 碳酸酯水解酶
登录 查看组织和合同定价。
选择尺寸
变更视图
关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-576-6
MDL number:
Specific activity:
≥2,000 W-A units/mg protein
Biological source:
bovine erythrocytes
biological source
bovine erythrocytes
Quality Level
form
lyophilized powder
specific activity
≥2,000 W-A units/mg protein
mol wt
30 kDa
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
Application
牛红血球(BCA)来源碳酸酐酶已用于研究去除酶结合金属离子Zn2+对酶的聚集行为的影响。使用螯合剂（例如EDTA（乙二胺四乙酸） ）去除金属离子，增强了酶采用熔融球状态的倾向。人们发现，这种状态的酶可与分子伴侣样α-晶状体蛋白结合并防止聚集。来自Sigma的酶已固定在电化学传感器上，以便开发出彻底溶解CO2的分析装置。其还用于BCA构象变化的热力学分析。
Biochem/physiol Actions
碳酸酐酶是一种锌金属酶，其分子量约为30,000Da。该酶催化二氧化碳水合成碳酸。它参与重要的生理和病理过程，如pH和CO2稳态、碳酸氢盐和CO2的转运、生物合成反应、骨吸收、钙化和致肿瘤性。因此，该酶是用于各种病理学（如青光眼、癫痫和帕金森病）的临床应用的抑制剂的重要靶标。
Other Notes
在0°C条件下，一Wilbur-Anderson (W-A)单位可使0.02M Trizma缓冲液pH在一分钟内从8.3降至 6.3。（一W-A单位基本上相当于一Roughton-Booth单位。）
Still not finding the right product?
Explore all of our products under 碳酸酐酶 来源于牛红细胞
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
动植物源性产品
此项目有
实验方案
Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)
Fraser J Moss et al.
The Journal of physiology, 598(24), 5821-5856 (2020-09-25)
According to the HCO 3 - metabolon hypothesis, direct association of cytosolic carbonic anhydrases (CAs) with the electrogenic Na/HCO3 cotransporter NBCe1-A speeds transport by regenerating/consuming HCO 3 - . The present work addresses published discrepancies as to whether
Use of carbonic anhydrase in electrochemical biosensors for dissolved CO2
Cammaroto C, et al.
Sensors and Actuators B, Chemical, 48(1-3), 439-447 (1998)
Mikhail Krasavin et al.
Journal of enzyme inhibition and medicinal chemistry, 35(1), 165-171 (2019-11-23)
Testing of an expanded, 800-compound set of analogues of the earlier described Strecker-type α-aminonitriles (selected from publicly available Enamine Ltd. Screening Collection) in thermal shift assay against bovine carbonic anhydrase (bCA) led to further validation of this new class of
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| C3934-100MG | 04061833486665 |
| C3934-500MG | 04061833486696 |