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安全信息

C-39325

Sigma-Aldrich

Fibroblast Growth Medium 2

SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

别名:

Recommended with C-23220

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.81

表单

aqueous solution

包装

pkg of 1 vial

技术

cell culture | mammalian: suitable

运输

dry ice

储存温度

−20°C

一般描述

Fibroblast Growth Medium 2 SupplementMix (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-23220

应用

Fibroblast Growth Medium 2 is a low-serum medium and has been developed for the in vitro cultivation of adult Human Fibroblasts. The medium is optimized for primary human cells, but can also be used for bovine, porcine, and rat fibroblasts, as well as fibroblast cell lines.Recommended for:Normal Human Dermal Fibroblasts (NHDF), adult donorHuman Pulmonary Fibroblasts (HPF)Human Aortic Adventitial Fibroblasts (HAoAF)Human Uterine Fibroblasts (HUF)

组分

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

质量

All lots of PromoCell Fibroblast Growth Medium 2 are subjected to comprehensive quality control tests using primary human adult fibroblasts. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested adult fibroblasts. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

制备说明

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.02 ml / mlBasic Fibroblast Growth Factor (recombinant human): 1 ng / ml Insulin: 5 µg / ml

储存及稳定性

Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

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价格

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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商品

Primary human fibroblasts and growth medium for the in vitro cultivation of juvenile and adult fibroblasts. Protocols for handling, cell passaging, media, and product use.

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