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表单
liquid
包装
pkg of 1 kit
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−20°C
一般描述
Fibroblast Growth Medium SupplementPack (basal medium sold separately)
To form a complete medium, it should be combined with the basal medium: C-23210
To form a complete medium, it should be combined with the basal medium: C-23210
应用
Fibroblast Growth Medium is a serum-free medium optimized for the cultivation of neonatal and juvenile fibroblasts. The medium is optimized for primary human cells, but can also be used for bovine, porcine, and rat fibroblasts as well as fibroblast cell lines.Recommended for:Normal Human Dermal Fibroblasts (NHDF), juvenile foreskin
组分
Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials
质量
All lots of PromoCell Fibroblast Growth Medium are subjected to comprehensive quality control tests using primary human fibroblasts. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested fibroblasts. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
制备说明
Final supplement concentrations after addition to the basal medium:Basic Fibroblast Growth Factor (recombinant human): 1 ng / ml Insulin: 5 µg / ml
储存及稳定性
Store the SupplementPack at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.
传代培养常规
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储存分类代码
10 - Combustible liquids
法规信息
新产品
商品
Primary human fibroblasts and growth medium for the in vitro cultivation of juvenile and adult fibroblasts. Protocols for handling, cell passaging, media, and product use.
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