应用
羧肽酶Y具有广泛的特异性并且在尿素中稳定。因此,该酶与其他羧肽酶不同,可以用于序列分析。由于其酰胺酶作用,该酶可用于具有酰胺化COOH-末端基团的肽,例如催产素和加压素的序列分析。
生化/生理作用
糖蛋白的分子量约为61,000,氮含量为12.74%。它是一个含有442个残基的单条多肽链,在碳水化合物部分含有16个葡糖胺残基。赖氨酸位于NH2末端,-Asp-Ser-Thr-Leu位COOH末端序列。该酶的主要作用是从多肽链上去除COOH末端残基。它可用作液泡标记酶,用于蛋白质转运和定位研究。
包装
包装尺寸基于蛋白质含量
单位定义
基于EM/230 = 191.5,在25°C,pH 6.75的条件下,一个单位每分钟可将1.0 mole μN-CBZ-Phe-Ala水解为N-CBZ-L-苯丙氨酸和L-丙氨酸。
外形
含有柠檬酸的冻干粉末。
分析说明
可能存在酰胺酶和酯酶活性
警示用语:
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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