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Merck
CN

C3888

Sigma-Aldrich

羧肽酶 Y 来源于面包酵母(酿酒酵母

lyophilized powder, ≥50 units/mg protein

别名:

丝氨酸羧肽酶, 肽基-L-氨基酸水解酶

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About This Item

CAS号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

等级

Proteomics Grade

质量水平

形式

lyophilized powder

比活

≥50 units/mg protein

分子量

61 kDa

组成

Protein, ≥75% E1%/280

运输

wet ice

储存温度

−20°C

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相关类别

应用

羧肽酶Y具有广泛的特异性并且在尿素中稳定。因此,该酶与其他羧肽酶不同,可以用于序列分析。由于其酰胺酶作用,该酶可用于具有酰胺化COOH-末端基团的肽,例如催产素和加压素的序列分析。

生化/生理作用

糖蛋白的分子量约为61,000,氮含量为12.74%。它是一个含有442个残基的单条多肽链,在碳水化合物部分含有16个葡糖胺残基。赖氨酸位于NH2末端,-Asp-Ser-Thr-Leu位COOH末端序列。该酶的主要作用是从多肽链上去除COOH末端残基。它可用作液泡标记酶,用于蛋白质转运和定位研究。

包装

包装尺寸基于蛋白质含量

单位定义

基于EM/230 = 191.5,在25°C,pH 6.75的条件下,一个单位每分钟可将1.0 mole μN-CBZ-Phe-Ala水解为N-CBZ-L-苯丙氨酸和L-丙氨酸。

外形

含有柠檬酸的冻干粉末。

分析说明

可能存在酰胺酶和酯酶活性

象形图

Health hazardExclamation mark

警示用语:

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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