生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen ~55 kDa
种属反应性
rat, human
种属反应性（根据同源性预测）
mouse
增强验证
recombinant expression
Learn more about Antibody Enhanced Validation
浓度
~1.0 mg/mL
技术
western blot: 0.5-1.0 μg/mL using HEK-293T cells expressing human calsequestrin-2
western blot: 0.5-1.0 μg/mL using extract of rat cardiac muscle (S1 fraction)
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... CASQ2(845)
mouse ... Casq2(12373)
rat ... Casq2(29209)
相关类别
一般描述
Calsequestrin is a major Ca2+ binding protein in cardiac and skeletal muscle.
Calsequestrin-2 (55 kDa) is found in both sarcoplasmic reticulum′s (SR) terminal cisternae luminal space of both cardiac and in slow skeletal muscle cells. It is made of three domains each with negatively charged thioredoxin-like folds with a β-sheet surrounded by four α-helices.
免疫原
synthetic peptide corresponding to amino acids 57-74 of rat calsequestrin-2, conjugated to KLH. This sequence is highly conserved in human and rat calsequestrin-2 (single amino acid substitution). This sequence is not found in calsequestrin-1.
应用
Anti-Calsequestrin-2 antibody has been used in immunoblotting, western blotting.
Anti-Calsequestrin-2 is suitable for western blot at a concentration of 0.5-1.0μg/mL using HEK-293T cells expressing human calsequestrin-2 and western blot at a concentration of 0.5-1.0μg/mL using extract of rat cardiac muscle (S1 fraction)
生化/生理作用
Over expression of cardiac calsequestrin (CASQ2) is associated with depressed cardiovascular function and hypertrophy. Mutations in CASQ2 are thought to be associated with catecholaminergic polymorphic ventricular tachycardia (CPVT). It is a high-capacity, low-affinity Ca2+ binding glycoprotein, which functions as an internal Ca2+ store in the lumen of the sarcoplasmic reticulum (SR).
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
Kathy Ye Morgan et al.
Journal of tissue engineering and regenerative medicine, 11(2), 342-353 (2014-06-12)
Mechanical stimulation has been used extensively to improve the function of cardiac engineered tissue, as it mimics the physical environment in which the tissue is situated during normal development. However, previous mechanical stimulation has been carried out under a constant
Absence of calsequestrin 2 causes severe forms of catecholaminergic polymorphic ventricular tachycardia
Postma A V, et al.
Circulation Research, 91(8), e21-e26 (2002)
Calsequestrin 2 and arrhythmias
Faggioni M and Knollmann B C.
American Journal of Physiology. Heart and Circulatory Physiology, 302(6), H1250-H1260 (2011)
Kathy Ye Morgan et al.
Tissue engineering. Part A, 20(11-12), 1654-1667 (2014-01-15)
Electrical and mechanical stimulation have both been used extensively to improve the function of cardiac engineered tissue as each of these stimuli is present in the physical environment during normal development in vivo. However, to date, there has been no
Calsequestrins in skeletal and cardiac muscle from adult Danio rerio
Furlan S, et al.
Journal of Muscle Research and Cell Motility, 37(1-2), 27-39 (2016)
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