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C3667

软骨素酶 ABC 来源于普通变形杆菌

BSA free, lyophilized powder, specific activity 50-250 units/mg protein

别名:

4.2.2.20, 4.2.2.21, Chondroitin ABC Lyase

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关于此项目

化学文摘社编号:
9024-13-9
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-777-9
MDL number:
MFCD00130795
EC 号:
4.2.2.4 (BRENDA, IUBMB)
Specific activity:
50-250 units/mg protein
Biological source:
bacterial (Proteus vulgaris)
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biological source

bacterial (Proteus vulgaris)

Quality Level

300

conjugate

(Glucosaminoglycan)

form

lyophilized powder

specific activity

50-250 units/mg protein

mol wt

120 kDa by gel filtration

composition

Protein, ~10% Lowry

solubility

0.01% bovine serum albumin aqueous (BSA) solution: soluble

application(s)

diagnostic assay manufacturing

foreign activity

protease, essentially free

storage temp.

−20°C

Application

该酶已被用于研究采用成年大鼠脑细胞，硫酸软骨素蛋白多糖所诱导的发急性、长期变化的后果。以新鲜牛角膜基质为原料，采用软骨素酶ABC对氨基聚糖进行酶解，测定了其在角质细胞培养中的含量。

Biochem/physiol Actions

硫酸软骨素酶ABC催化含有(1-4)-β-D-己糖胺基和(1-3)-β-葡聚糖或(1-3)-α-葡聚糖-L-iduronosyl与含有4-脱氧-β-D-葡聚糖-4-enuronosyl基团的双糖链接的清除性降解。它作用于4-硫酸软骨素、6-硫酸软骨素和皮肤硫酸软骨素，缓慢作用于透明质酸盐。分子量约为120 kDa，含有2个不相同的亚基，分子量分别为86 kDa和32 kDa。找到的最佳pH值与硫酸软骨素为8.0，与透明质酸为6.8，以及最佳温度是37°C。以1 mM Zn2+ 为抑制剂，0.05 M醋酸盐为酶的激活剂。

Packaging

基于硫酸软骨素 C活性包装。

Preparation Note

亲和纯化

Other Notes

包含磷酸盐缓冲液和稳定剂
在37°C、pH 8.0条件下，一单位每分钟将释放1.0 μmole的2-乙酰氨基-2-脱氧-3-O-(β-D-葡萄糖)-4-烯-吡喃糖醛糖醛酸）-4-O-磺基-D-半乳糖的混合物和1.0 μmole来自鲨鱼软骨硫酸软骨素的2-乙酰氨基-2-脱氧-3-O-(β-D-葡萄糖-4-烯-吡喃糖醛糖醛酸)-6-O-磺基-D-半乳糖。
有关复合碳水化合物分析酶的更多信息，请见www.sigma-aldrich.com/enzymeexplorer

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存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000533898
0000533896
0000533898
0000533896
0000442821

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P N Bansal et al.
Osteoarthritis and cartilage, 18(2), 184-191 (2009-10-10)
An early hallmark of osteoarthritis (OA) is the progressive loss of glycosaminoglycans (GAGs), the extracellular matrix (ECM) component of articular cartilage that confers it with compressive stiffness. Our aim in this work is to establish the feasibility of using Contrast
Susan M Smith et al.
International journal of molecular sciences, 20(3) (2019-01-27)
The aim of this study was to assess if the ovine articular cartilage serine proteinase inhibitors (SPIs) were related to the Kunitz inter-α-trypsin inhibitor (ITI) family. Ovine articular cartilage was finely diced and extracted in 6 M urea and SPIs
Elizabeth J Bradbury et al.
Brain research bulletin, 84(4-5), 306-316 (2010-07-14)
Chondroitin sulphate proteoglycans (CSPGs) are potent inhibitors of growth in the adult CNS. Use of the enzyme chondroitinase ABC (ChABC) as a strategy to reduce CSPG inhibition in experimental models of spinal cord injury has led to observations of a
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全球贸易项目编号

货号GTIN
C3667-10UN04061833486214
C3667-5UN04061833486221