等级
for molecular biology
质量水平
检测方案
≥99.0%
形式
powder
mp
176 °C (dec.) (lit.)
溶解性
H2O: 294 mg/mL, clear, colorless
异质活性
DNase, RNase, protease, none detected
SMILES字符串
O.O.Cl[Ca]Cl
InChI
1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2
InChI key
LLSDKQJKOVVTOJ-UHFFFAOYSA-L
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应用
氯化钙二水合物用于：
制备合格大肠杆菌用于转化（118）
用于制备聚磷酸钙纳米球和诱导碱性磷酸酶(119)
复性缓冲液的成分，用于金黄色葡萄球菌粗酶提取物（120）酶谱
制备合格大肠杆菌用于转化（118）
用于制备聚磷酸钙纳米球和诱导碱性磷酸酶(119)
复性缓冲液的成分，用于金黄色葡萄球菌粗酶提取物（120）酶谱
生化/生理作用
氯化钙与质粒一起用于微生物的热休克转化。带正电的钙离子与DNA磷酸盐和脂多糖（LPS）中带负电的磷酸化脂质A形成阳离子桥，有助于DNA转化进微生物。
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
Preparation of calcium competent Escherichia coli and heat-shock transformation
Chang AY, et al.
Journal of Experimental Microbiology and Immunology, 1, 22-25 (2017)
Bradley T Estes et al.
Nature protocols, 5(7), 1294-1311 (2010-07-03)
The ability to isolate, expand and differentiate adult stem cells into a chondrogenic lineage is an important step in the development of tissue engineering approaches for cartilage repair or regeneration for the treatment of joint injury or osteoarthritis, as well
Yikun Mei et al.
Frontiers in microbiology, 11, 996-996 (2020-06-26)
Due to the increasing prevalence of pathogenic fungal infections, the emergence of antifungal resistant clinical isolates worldwide, and the limited arsenal of available antifungals, developing new antifungal strategies is imperative. In this study, we screened a library of 1068 FDA-approved
Hui Sun Leong et al.
Nature communications, 5, 3947-3947 (2014-05-24)
Non-coding RNAs (ncRNAs) are frequent and prevalent across the taxa. Although individual non-coding loci have been assigned a function, most are uncharacterized. Their global biological significance is unproven and remains controversial. Here we investigate the role played by ncRNAs in
Zhijian Zhao et al.
Scientific reports, 10(1), 4399-4399 (2020-03-12)
The retina decomposes visual stimuli into parallel channels that encode different features of the visual environment. Central to this computation is the synaptic processing in a dense layer of neuropil, the so-called inner plexiform layer (IPL). Here, different types of
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