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物料
dextran beads
无菌性
non-sterile
粒径
>175 μm (spherical)
密度
1.04 g/cm3 at 25 °C
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一般描述
体外难培养细胞的首选微载体。
微载体培养是一种使贴壁依赖性细胞的高产培养成为可能的实用技术。Cytodex是专门为培养各种动物细胞而开发的，培养量从几毫升到6000多升不等。在简单的悬浮培养系统中使用Cytodex可得到每毫升数百万个细胞的产量。
通过将一层薄薄的变性胶原蛋白与交联葡聚糖基质化学偶联而形成，这是体外难培养细胞的首选微载体，特别是具有上皮样形态的细胞。
微载体培养是一种使贴壁依赖性细胞的高产培养成为可能的实用技术。Cytodex是专门为培养各种动物细胞而开发的，培养量从几毫升到6000多升不等。在简单的悬浮培养系统中使用Cytodex可得到每毫升数百万个细胞的产量。
通过将一层薄薄的变性胶原蛋白与交联葡聚糖基质化学偶联而形成，这是体外难培养细胞的首选微载体，特别是具有上皮样形态的细胞。
表面包被变性猪皮胶原的葡聚糖珠。
应用
Cytodex™3微载体珠适用于：
- 细胞的分离、运输和储存
- 体外难培养的细胞，特别是具有上皮形态、敏感、分化或铺板效率低的细胞（例如内分泌细胞、肝细胞）
- 病毒、细胞和重组细胞产品（如激素、核酸、酶、干扰素）的大规模生产
对于在培养体系中通常难以生长的细胞（特别是上皮来源的细胞）。
特点和优势
- 专为培养原代细胞或具有上皮样形态的细胞而设计，特别是铺板效率低的细胞或通常难以生长的分化或敏感细胞，例如肝细胞、内分泌细胞。
- 微孔基质表面有一层酸变性猪胶原蛋白（明胶）。
- 旨在产生具有最高活力的收获细胞。
- 透明，便于对附着的细胞进行显微镜检查。
制备说明
使用前，将微载体珠在PBS（50mL/g）中溶胀，并在120℃下高压灭菌。
法律信息
Cytodex is a registered trademark of Cytiva
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
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