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物料
dextran beads
无菌性
non-sterile
粒径
>175 μm (spherical)
密度
1.04 g/cm3 at 25 °C
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一般描述
表面包被变性猪皮胶原的葡聚糖珠。
The microcarrier of choice for cells that may be difficult to culture in vitro.
Microcarrier culture is a technique which makes possible the practical high yield culture of anchorage-dependent cells. Cytodex has been specifically developed for the culture of a wide range of animal cells in culture volumes ranging from a few ml to more than 6000 L. Using Cytodex in simple suspension culture systems provides yields of several million cells per mL.
Formed by chemically coupling a thin layer of denatured collagen to the cross-linked dextran matrix, this is the microcarrier of choice for cells that may be difficult to culture in vitro, and particularly for cells with an epithelial like morphology.
Microcarrier culture is a technique which makes possible the practical high yield culture of anchorage-dependent cells. Cytodex has been specifically developed for the culture of a wide range of animal cells in culture volumes ranging from a few ml to more than 6000 L. Using Cytodex in simple suspension culture systems provides yields of several million cells per mL.
Formed by chemically coupling a thin layer of denatured collagen to the cross-linked dextran matrix, this is the microcarrier of choice for cells that may be difficult to culture in vitro, and particularly for cells with an epithelial like morphology.
应用
Cytodex™3 microcarrier beads are suitable for:
- the isolation, transportation, and storage of cells
- cells that might be challenging to culture in vitro, especially cells with an epithelial morphology, sensitive, differentiated, or low in plating efficiency (e.g., endocrine cells, hepatocytes)
- large-scale production of viruses, cells, and recombinant cell products (e.g. hormones, nucleic acids, enzymes, interferon)
对于在培养体系中通常难以生长的细胞（特别是上皮来源的细胞）。
特点和优势
- Designed for culture of primary cells or those with an epithelioid morphology, especially cells with low plating-efficiency or differentiated or sensitive cell-types which are normally difficult to grow, e.g. hepatocytes, endocrine cells.
- Contains a layer of acid-denatured porcine collagen (gelatin) on the surface of the microporous matrix.
- Designed to yield harvested cells of the highest viability.
- Transparent for easy microscopic examination of attached cells.
制备说明
使用前，将微载体珠在PBS（50mL/g）中溶胀，并在120℃下高压灭菌。
法律信息
Cytodex is a registered trademark of Cytiva
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
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