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安全信息

C2905

Sigma-Aldrich

软骨素酶 ABC 来源于普通变形杆菌

lyophilized powder, 0.3-3 units/mg solid

别名:

Chondroitin ABC Lyase

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About This Item

CAS号:
9024-13-9
EC 号:
4.2.2.4 (BRENDA, IUBMB)
EC 号:
232-777-9
MDL编号:
MFCD00130795
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

bacterial (Proteus vulgaris)

质量水平

300

偶联物

(Glucosaminoglycan)

表单

lyophilized powder

比活

0.3-3 units/mg solid

分子量

~120 kDa by gel filtration

组成

Protein, ~35% biuret

应用

diagnostic assay manufacturing

储存温度

−20°C

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相关类别

应用

Sigma的软骨素酶ABC被用来消化O连接的硫酸软骨素类糖胺聚糖(CSGAG)。该酶还被用来消化蛋白质多糖中的糖胺聚糖链，在哺乳动物系统中构建和表达重组的多功能蛋白聚糖基因。此外，它还被用来消化蛋白聚糖复合物中的硫酸软骨素。该酶还被用于从蛋白聚糖中去除糖链，从而消除蛋白聚糖的轴突生长抑制作用。

生化/生理作用

软骨苷酶ABC催化含有(1-4)-β-D-己糖氨基和(1-3)-β-D-葡萄糖醛酸基(或(1-3)-α-L-iduronosyl)的多糖与含有4-脱氧-β-D-葡萄糖-4-enuronosyl基团的双糖的清除降解。它作用于硫酸软骨素4、硫酸软骨素6、硫酸皮肤素。但是，它对透明质酸盐作用缓慢。其分子量约为120 kDa，含分子量分别为86 kDa和32 kDa的两个不同亚单位。硫酸软骨素pH值为8.0，透明质酸pH值为6.8。最佳温度为37℃。1mM Zn2+ 可作为其抑制剂，0.05 M乙酸为该酶的激活剂。

包装

包装按照软骨素酶C活性。

单位定义

在37°C、pH 8.0条件下，一单位每分钟将释放1.0 μmole的2-乙酰氨基-2-脱氧-3-O-(β-D-葡萄糖)-4-烯-吡喃糖醛糖醛酸）-4-O-磺基-D-半乳糖的混合物和1.0 μmole来自鲨鱼软骨硫酸软骨素的2-乙酰氨基-2-脱氧-3-O-(β-D-葡萄糖-4-烯-吡喃糖醛糖醛酸)-6-O-磺基-D-半乳糖。

外形

含 Tris 缓冲盐的冻干粉

制备说明

在0.01%牛血清白蛋白水溶液(BSA)中复溶。随后的稀释液可以制成pH 8.0的含有50mm Tris,60mm乙酸钠和0.02% BSA的缓冲液。溶液需新鲜配制。

其他说明

有关复合碳水化合物分析酶的更多信息，请见www.sigma-aldrich.com/enzymeexplorer

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
0000410704
0000410704
0000389744
0000365342
0000360197

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the molecular weight of this enzyme (Product No. C2905)?

    Chondroitinase ABC is a lyase with a reported molecular weight of approximately 120 kDa by gel filtration and is composed of two subunits with molecular weights of 86 and 32.

  6. What is the composition of Product No. C2905?

    This product consists of approximately 35% protein as determined by the Biuret method. BSA (bovine serum albumin) is a major component (~90%) of the protein. The remaining 65% is Tris buffer salts.

  7. How should Product No. C2905 be reconstituted?

    Reconstitute the product in a 0.01% bovine serum albumin aqueous solution. Subsequent dilutions can be made into a buffer containing 50 mM Tris, pH 8.0, with 60 mM sodium acetate and 0.02% bovine serum albumin. Solutions should be prepared fresh.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Y Zhang et al.
The Journal of biological chemistry, 273(33), 21342-21351 (1998-08-08)
Versican is a member of the large aggregating chondroitin sulfate proteoglycan family. We have expressed in NIH3T3 fibroblasts a recombinant versican mini-gene comprising the G1 and G3 domains and 15% of the CS domain. We observed that expression of the
Mafalda Resende et al.
International journal for parasitology, 39(11), 1195-1204 (2009-03-28)
Individuals living in areas with high Plasmodium falciparum transmission acquire immunity to malaria over time and adults have a markedly reduced risk of contracting severe disease. However, pregnant women constitute an important exception. Pregnancy-associated malaria is a major cause of
Chin Lik Tan et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 31(17), 6289-6295 (2011-04-29)
Chondroitin sulfate proteoglycans (CSPGs) are upregulated after CNS lesions, where they inhibit axon regeneration. In order for axon growth and regeneration to occur, surface integrin receptors must interact with surrounding extracellular matrix molecules. We have explored the hypothesis that CSPGs
Nicolett Kanyo et al.
Scientific reports, 10(1), 22422-22422 (2021-01-01)
The glycocalyx is thought to perform a potent, but not yet defined function in cellular adhesion and signaling. Since 95% of cancer cells have altered glycocalyx structure, this role can be especially important in cancer development and metastasis. The glycocalyx
Gion Fessel et al.
Matrix biology : journal of the International Society for Matrix Biology, 28(8), 503-510 (2009-08-25)
The glycosaminoglycan (GAG) dermatan sulfate and chondroitin sulfate side-chains of small leucine-rich proteoglycans have been increasingly posited to act as molecular cross links between adjacent collagen fibrils and to directly contribute to tendon elasticity. GAGs have also been implicated in
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