推荐产品
生物来源
rabbit
偶联物
unconjugated
抗体形式
IgG fraction of antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~75 kDa
种属反应性
human
技术
western blot: 1:250-1:500 using K-562 lysate
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... CPSF3(51692)
一般描述
Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9. It is encoded by the CASP3 gene. This gene is a member of the metallo-β-lactamase family and is referred as CPSF73 and CPSF-73. CPSF73 is a 73kDa subunit and is the pre-mRNA 3′-end-processing endonuclease.
应用
Anti-CPSF3 (C-terminal) antibody produced in rabbit is suitable for western blotting at a dilution of 1:250-1:500 using K-562 lysate.
生化/生理作用
CPSF3 is induced by interaction between CSR1 and CPSF3 and is translocated from the nucleus to the cytoplasm, resulting in inhibition of polyadenylation. Down-regulation of CPSF3 using small interfering RNA induces cell death. CPSF73 mediates cleavage coupled to polyadenylation and histone pre-mRNA processing. HIV-1 Tat protein interacts with CPSF-73 and counteracts its repressive activity on the HIV-1 LTR promoter. During the cleavage and polyadenylation, CPSF plays a central role in processing the reaction.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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法规信息
新产品
Z-H Zhu et al.
Oncogene, 28(1), 41-51 (2008-09-23)
CSR1 (cellular stress response 1), a newly characterized tumor-suppressor gene, undergoes hypermethylation in over 30% of prostate cancers. Re-expression of CSR1 inhibits cell growth and induces cell death, but the mechanism by which CSR1 suppresses tumor growth is not clear.
Corey R Mandel et al.
Nature, 444(7121), 953-956 (2006-11-28)
Most eukaryotic messenger RNA precursors (pre-mRNAs) undergo extensive maturational processing, including cleavage and polyadenylation at the 3'-end. Despite the characterization of many proteins that are required for the cleavage reaction, the identity of the endonuclease is not known. Recent analyses
A Jenny et al.
Science (New York, N.Y.), 274(5292), 1514-1517 (1996-11-29)
The 3' ends of most eukaryotic messenger RNAs are generated by endonucleolytic cleavage and polyadenylation. In mammals, the cleavage and polyadenylation specificity factor (CPSF) plays a central role in both steps of the processing reaction. Here, the cloning of the
Laureano de la Vega et al.
Journal of molecular biology, 372(2), 317-330 (2007-08-03)
Gene expression in eukaryotes requires the post-transcriptional cleavage of mRNA precursors into mature mRNAs. The cleavage and polyadenylation specificity factor (CPSF) is critical for this process and its 73 kDa subunit (CPSF-73) mediates cleavage coupled to polyadenylation and histone pre-mRNA
Lee Davidson et al.
Genes & development, 28(4), 342-356 (2014-01-31)
3' end formation of pre-mRNAs is coupled to their transcription via the C-terminal domain (CTD) of RNA polymerase II (Pol II). Nearly all protein-coding transcripts are matured by cleavage and polyadenylation (CPA), which is frequently misregulated in disease. Understanding how
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