生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
hCP, monoclonal
分子量
antigen 34 kDa
包含
15 mM sodium azide
种属反应性
rat, rabbit, pig, human, mouse
技术
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:10,000 using human uterus extract
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... CNN1(1264) , CNN2(1265)
mouse ... Cnn1(12797) , Cnn2(12798)
rat ... Cnn1(65204) , Kcnip4(259243)
特异性
hCP抗体（又称CALP）可定位哺乳动物平滑肌中的钙调蛋白。在人子宫中，一种约 27 kDa 的附加条带(1-钙调蛋白)也可能随之染色。该抗体不会与骨骼肌、心脏或非肌肉组织的钙调蛋白发生交叉反应。然而，该抗体与人或小鼠骨骼肌中的表位(150 kDa 范围)表现出交叉反应。在免疫组化染色中，该产品表现出平滑肌特异性。它可以染色组织切片和原代培养细胞(或早期传代细胞)中的血管和内脏平滑肌细胞，但不能染色大多数最初源自平滑肌的细胞系。该抗体不能染色上皮、内皮或结缔组织成纤维细胞。 该产品不与鸡肉的平滑肌组织发生交叉反应。
免疫原
人子宫平滑肌提取物。
应用
小鼠产生的抗-钙调蛋白单克隆抗体适用于以下应用：
- 使用福尔马林固定、石蜡包埋切片的免疫组化
- 免疫沉淀
- 微阵列
- 蛋白质印迹（使用人子宫提取物，稀释度为 1:10,000）
- 流式细胞仪分析
生化/生理作用
钙调蛋白是钙结合蛋白，属于肌动蛋白相关蛋白家族。钙调蛋白是血管平滑肌(VSM)中蛋白激酶 C(PKC)自磷酸化所必需的。钙调蛋白-1 抑制可防止子宫平滑肌细胞迁移、引起形态变化和F-肌动蛋白重排，而不会影响其增殖和凋亡。钙调蛋白 H1(CN)是平滑肌细胞的分化标志物，在几种人类肿瘤的血管中下调。它可以抑制肌动球蛋白 ATP 酶活性。h1 和 h2 钙调蛋白与 F-肌动蛋白结合，并在调节平滑肌和非肌肉细胞的肌动蛋白丝中发挥关键作用。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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