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Merck
CN

C2409

Sigma-Aldrich

肌酸酶 来源于放线杆菌

lyophilized powder, ≥4 units/mg solid

别名:

肌酸脒基水解酶

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

bacterial (Actinobacillus spp.)

质量水平

表单

lyophilized powder

比活

≥4 units/mg solid

分子量

~100 kDa

储存温度

−20°C

一般描述

肌酸酶是催化肌酸水解的同型二聚体。它由两个单体亚基和两个指定结构域组成;N和C末端结构域。C端折叠在两个结构相似的结构域内,同时具有α螺旋和反平行β折叠。在这两个结构域之间,一个巯基作为活性位点,其活性无金属依赖性。

应用

肌酸酶与肌氨酸氧化酶混合可用于测定不同pH值、温度、酶比和缓冲液浓度下的肌酸水平。也可以通过使用离心分析仪来测定血浆肌酐水平。

生化/生理作用

肌酸酶加速肌酸和水分子转化形成肌氨酸和尿素的反应。它总是以同型二聚体状态起作用,并由氯化胆碱诱导。

物理属性

等电点: 4.6 ± 0.1
米氏常数: 1.9 x 10‾2M(肌酸)
结构: 每摩尔酶2个亚基
抑制剂: Cu++,Hg++,Ag+
最佳pH: 8.0
最佳温度: 40℃
pH稳定性: pH 5.5 − 9.0(25℃,16hr)
热稳定性: 低于50℃(pH 7.5,30分钟)

单位定义

在pH 7.5、37℃条件下,1个单位将每分钟水解1.0 μmol肌酸形成尿素和肌氨酸。

外形

含有糖和EDTA 作为稳定剂的冻干粉

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

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分析证书(COA)

Lot/Batch Number

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访问文档库

Balasundaram Padmanabhan et al.
Acta crystallographica. Section D, Biological crystallography, 58(Pt 8), 1322-1328 (2002-07-24)
The crystal structure of Actinobacillus creatine amidinohydrolase has been solved by molecular replacement. The amino-acid sequence has been derived from the crystal structure. Crystals belong to space group I222, with unit-cell parameters a = 111.26 (3), b = 113.62 (4)
J F Bazan et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(7), 2473-2477 (1994-03-29)
Amino acid sequence comparison suggests that the structure of Escherichia coli methionine aminopeptidase (EC 3.4.11.18) and the C-terminal domain of Pseudomonas putida creatinase (EC 3.5.3.3) are related. A detailed comparison of the three-dimensional folds of the two enzymes confirms this
H Crocker et al.
Journal of clinical pathology, 41(5), 576-581 (1988-05-01)
An enzymatic kit method for the determination of plasma creatinine was optimised for use with a centrifugal analyser and its performance characteristics and practicability compared with an end point and a kinetic Jaffé-based method. The enzymatic method exhibited several advantages
Agnieszka Dzikowska et al.
Fungal genetics and biology : FG & B, 38(2), 175-186 (2003-03-07)
The arginine catabolism gene otaA encoding ornithine transaminase (OTAse) is specifically induced by arginine and is under the control of the broad-domain carbon and nitrogen repression systems. Arginine induction is mediated by a product of arcA gene coding for Zn(2)C(6)
N A Siddiqi et al.
Saudi journal of kidney diseases and transplantation : an official publication of the Saudi Center for Organ Transplantation, Saudi Arabia, 17(2), 203-207 (2006-08-15)
Polyoma virus nephropathy (BK virus) is being recognized as an important cause of graft failure. It is usually confused with acute rejection. No cases have been reported from the kingdom of Saudi Arabia. We report a case of a Saudi

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