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Merck
CN

C2273

Sigma-Aldrich

碳酸酐酶 来源于牛红细胞

For use as a marker in SDS-PAGE

别名:

碳酸盐脱水酶, 碳酸酯水解酶

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

bovine erythrocytes

质量水平

表单

powder

分子量

~29 kDa

包装

vial of 5 mg

储存温度

2-8°C

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一般描述

碳酸酐酶是一种含锌金属酶。碳酸酐酶的活性中心含有三个保守的组氨酸残基(His94、His96和His119)。

应用

碳酸酐酶已被用于:
  • 作为9种蛋白混合物的组分之一,用于牛脑样品的比较蛋白质组学质谱分析
  • 作为凝胶过滤色谱的分子标志物
  • 作为测定CMA/20微透析探针回收率的蛋白标准品

生化/生理作用

碳酸酐酶是二氧化碳可逆水合过程的必需因子。它在呼吸、离子转运、酸碱平衡、脂质和糖类代谢通路等生理过程中也起重要作用。碳酸酐酶还参与电解质分泌和二氧化碳固定。

单位定义

一个胶原蛋白消化单元 (CDU) 从牛跟腱胶原蛋白中释放的肽链量和1.0 μmole亮氨酸在37℃下、pH7.4中5小时所得茚三酮颜色相同。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

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分析证书(COA)

Lot/Batch Number

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Md Imtaiyaz Hassan et al.
Bioorganic & medicinal chemistry, 21(6), 1570-1582 (2012-05-23)
The carbonic anhydrases enzymes (CAs, EC 4.2.1.1) are zinc containing metalloproteins, which efficiently catalyse the reversible conversion of carbon dioxide to bicarbonate and release proton. These enzymes are essentially important for biological system and play several important physiological and patho-physiological
Alan J Rosenbloom et al.
Journal of neuroscience methods, 148(2), 147-153 (2005-07-27)
Unlike all other currently available sampling techniques, microdialysis allows the continuous recovery of dialysed fluid from the interstitial space of living tissue. Microdialysis has been extensively used to study small molecules such as neurotransmitters, metabolites and drugs in the brain
Arun Devabhaktuni et al.
Journal of proteome research, 15(3), 732-742 (2016-01-09)
Dependent on concise, predefined protein sequence databases, traditional search algorithms perform poorly when analyzing mass spectra derived from wholly uncharacterized protein products. Conversely, de novo peptide sequencing algorithms can interpret mass spectra without relying on reference databases. However, such algorithms
Joana da Costa Ores et al.
Chemosphere, 88(2), 255-259 (2012-04-20)
This work presents a study of industrially applicable techniques to obtain a biologically supported carbon dioxide capture system, based on the extraction of carbonic anhydrase from bovine blood. Carbonic anhydrase is a metalloenzyme which catalyzes the reversible hydration of carbon
Fatima Gasset-Rosa et al.
Neuron, 102(2), 339-357 (2019-03-12)
While cytoplasmic aggregation of TDP-43 is a pathological hallmark of amyotrophic lateral sclerosis and frontotemporal dementia, how aggregates form and what drives its nuclear clearance have not been determined. Here we show that TDP-43 at its endogenous level undergoes liquid-liquid

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