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磷酸纤维素用于蛋白质层析、离子交换层析和阳离子交换介质。已研究磷酸纤维素 (CELLPHOS) 作为从水样溶液中分析预富集痕量 Cd (II)、Cr (III)、Cu (II) 和 Ni (II) 的收集器，并用于纯化和表征考古发掘中约 3000 年前人骨的酸性磷酸酶。
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个人防护装备
Eyeshields, Gloves, type N95 (US)
A N Carter
Current protocols in molecular biology, Chapter 18, Unit 18-Unit 18 (2008-02-12)
In studies of the regulation of specific biochemical events by reversible phosphorylation, assaying the protein kinases themselves can often lead to significant progress in understanding the mechanistic details of a system under study. This unit describes assays for a variety
[Isolation and purification of nonspecific nuclease of cyanobacterium Plectonema boryanum CALU 465].
N V Tsymbal et al.
Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993), 66(1), 29-35 (2004-04-24)
Nonspecific nuclease has been isolated from the cells of cyanobacterium Plectonema boryanum and purified to homogenic state. It has been established that the method of centrifugation of cell-free culture extract in the sucrose density gradient is efficient for the separation
Yaşar Demir et al.
Preparative biochemistry & biotechnology, 33(4), 311-320 (2003-11-11)
In this research, acid phosphatase was purified and characterized from approximately 3000-year-old human bones from archeological excavations. Using anion exchange chromatography, two isoenzymes, TrACP and TsACP, were isolated from the bone. TrACP and TsACP were eluted separately, with a concentration
Overexpression and purification of Saccharomyces cerevisiae DNA topoisomerase II from yeast.
J E Lindsley
Methods in molecular biology (Clifton, N.J.), 94, 187-197 (2003-07-08)
Atanaska V Mitkova et al.
Biochemistry, 41(16), 5255-5265 (2002-04-17)
Plasmid DNA replication in nuclear extracts of Saccharomyces cerevisiae in vitro has been shown to be S-phase specific, similar to that observed in vivo. We report here a reconstituted in vitro system with partially purified replication proteins, purified replication protein
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