跳转至内容
Merck
CN

C1403

Sigma-Aldrich

胆固醇酯酶 来源于假单胞菌

lyophilized powder, ≥200,000 units/g protein

别名:

胆固醇酯酶 来源于荧光假单胞菌, 甾醇酯酰水解酶

登录查看公司和协议定价


About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

表单

lyophilized powder

质量水平

比活

≥200,000 units/g protein

分子量

~300 kDa

组成

protein, ≥40% biuret

储存温度

−20°C

正在寻找类似产品? 访问 产品对比指南

应用

当在临床分析中与胆固醇氧化酶结合使用时,该酶可用于酶法测定总胆固醇。

生化/生理作用

胆固醇酯酶(CE)是一种可逆酶,可以水解或合成胆固醇和其他固醇的脂肪酸酯。水不溶性长链脂肪酸酯的水解需要经过胆盐活化。短链脂肪酸和溶血磷脂的水溶性酯的水解则不需要胆盐的活化。它还可水解三、二和单酰基甘油、磷脂、溶血磷脂和神经酰胺。该酶在脂质和脂蛋白代谢以及动脉粥样硬化中可能具有多种功能。

物理属性

稳定性:在–20°C稳定至少一年
等电点:5.9 ± 0.1
米氏常数:5.4 x 10‾5M(亚油酸脂),6.6 x 10‾5M(油酸)
3.7 x 10‾5M(亚油酸脂),1.5 x 10‾4M(棕榈酸)
1.2 x 10‾4M(肉豆蔻),2.3 x 10‾5M(硬脂酸酯)
抑制剂:Hg++、Ag+、离子型去垢剂
最适pH值: 7.0 − 9.0
最适温度:40°C
pH稳定性: pH 5.0 − 9.0 (25°C, 24hr)
热稳定性:低于55°C(pH 7.5,10分钟)

单位定义

在牛磺胆酸盐存在下,一个单位在 pH7.0,37°C 下每分钟将 1.0μmole胆固醇油酸酯水解成胆固醇和油酸。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

从最新的版本中选择一种:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

David Y Hui et al.
Journal of lipid research, 43(12), 2017-2030 (2002-11-28)
Carboxyl ester lipase (CEL), previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The active site catalytic triad of serine-histidine-aspartate is centrally
Javier Sánchez-Marco et al.
Biomedicines, 10(3) (2022-03-26)
Thioredoxin domain containing 5 (TXNDC5) is a protein disulfide isomerase involved in several diseases related to oxidative stress, energy metabolism and cellular inflammation. In a previous manuscript, a negative association between fatty liver development and hepatic Txndc5 expression was observed.
Farid Shokry Ataya
Molecules (Basel, Switzerland), 17(9), 10399-10413 (2012-09-01)
Acid lipase belongs to a family of enzymes that is mainly present in lysosomes of different organs and the stomach. It is characterized by its capacity to withstand acidic conditions while maintaining high lipolytic activity. We cloned for the first
Pornpen Srisawasdi et al.
Journal of clinical laboratory analysis, 26(6), 420-430 (2012-11-13)
Accurate determination of cholesterol requires complete hydrolysis of cholesteryl esters and must be very fast for the kinetic cholesterol assay. We investigated the properties of cholesterol esterase derived from Pseudomonas fluorescens, Candida cylindracea, bovine pancreas, and porcine pancreas for cholesterol
Ju-Hee Lee et al.
Biochemical and biophysical research communications, 426(4), 492-497 (2012-09-18)
Sulforaphane, an aliphatic isothiocyanate derived from cruciferous vegetables, is known for its antidiabetic properties. The effects of sulforaphane on lipid metabolism in adipocytes are not clearly understood. Here, we investigated whether sulforaphane stimulates lipolysis. Mature adipocytes were incubated with sulforaphane

商品

胆固醇酯化可改善运输。胆固醇酯可更轻松地封装在脂蛋白内部-增加了在血液中可轻松运输的胆固醇数量。

Cholesterol esterification enhances transport efficiency in lipoproteins for increased blood stream transport.

实验方案

Protocol for Assay Procedure for Cholesterol Esterase

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门