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应用
当在临床分析中与胆固醇氧化酶结合使用时,该酶可用于酶法测定总胆固醇。
生化/生理作用
胆固醇酯酶(CE)是一种可逆酶,可以水解或合成胆固醇和其他固醇的脂肪酸酯。水不溶性长链脂肪酸酯的水解需要经过胆盐活化。短链脂肪酸和溶血磷脂的水溶性酯的水解则不需要胆盐的活化。它还可水解三、二和单酰基甘油、磷脂、溶血磷脂和神经酰胺。该酶在脂质和脂蛋白代谢以及动脉粥样硬化中可能具有多种功能。
物理属性
稳定性:在–20°C稳定至少一年
等电点:5.9 ± 0.1
米氏常数:5.4 x 10‾5M(亚油酸脂),6.6 x 10‾5M(油酸)
3.7 x 10‾5M(亚油酸脂),1.5 x 10‾4M(棕榈酸)
1.2 x 10‾4M(肉豆蔻),2.3 x 10‾5M(硬脂酸酯)
抑制剂:Hg++、Ag+、离子型去垢剂
最适pH值: 7.0 − 9.0
最适温度:40°C
pH稳定性: pH 5.0 − 9.0 (25°C, 24hr)
热稳定性:低于55°C(pH 7.5,10分钟)
等电点:5.9 ± 0.1
米氏常数:5.4 x 10‾5M(亚油酸脂),6.6 x 10‾5M(油酸)
3.7 x 10‾5M(亚油酸脂),1.5 x 10‾4M(棕榈酸)
1.2 x 10‾4M(肉豆蔻),2.3 x 10‾5M(硬脂酸酯)
抑制剂:Hg++、Ag+、离子型去垢剂
最适pH值: 7.0 − 9.0
最适温度:40°C
pH稳定性: pH 5.0 − 9.0 (25°C, 24hr)
热稳定性:低于55°C(pH 7.5,10分钟)
单位定义
在牛磺胆酸盐存在下,一个单位在 pH7.0,37°C 下每分钟将 1.0μmole胆固醇油酸酯水解成胆固醇和油酸。
警示用语:
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
从最新的版本中选择一种:
Journal of lipid research, 43(12), 2017-2030 (2002-11-28)
Carboxyl ester lipase (CEL), previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The active site catalytic triad of serine-histidine-aspartate is centrally
Biomedicines, 10(3) (2022-03-26)
Thioredoxin domain containing 5 (TXNDC5) is a protein disulfide isomerase involved in several diseases related to oxidative stress, energy metabolism and cellular inflammation. In a previous manuscript, a negative association between fatty liver development and hepatic Txndc5 expression was observed.
Molecules (Basel, Switzerland), 17(9), 10399-10413 (2012-09-01)
Acid lipase belongs to a family of enzymes that is mainly present in lysosomes of different organs and the stomach. It is characterized by its capacity to withstand acidic conditions while maintaining high lipolytic activity. We cloned for the first
Journal of clinical laboratory analysis, 26(6), 420-430 (2012-11-13)
Accurate determination of cholesterol requires complete hydrolysis of cholesteryl esters and must be very fast for the kinetic cholesterol assay. We investigated the properties of cholesterol esterase derived from Pseudomonas fluorescens, Candida cylindracea, bovine pancreas, and porcine pancreas for cholesterol
Biochemical and biophysical research communications, 426(4), 492-497 (2012-09-18)
Sulforaphane, an aliphatic isothiocyanate derived from cruciferous vegetables, is known for its antidiabetic properties. The effects of sulforaphane on lipid metabolism in adipocytes are not clearly understood. Here, we investigated whether sulforaphane stimulates lipolysis. Mature adipocytes were incubated with sulforaphane
商品
胆固醇酯化可改善运输。胆固醇酯可更轻松地封装在脂蛋白内部-增加了在血液中可轻松运输的胆固醇数量。
Cholesterol esterification enhances transport efficiency in lipoproteins for increased blood stream transport.
实验方案
Protocol for Assay Procedure for Cholesterol Esterase
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