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安全信息

C-12915

Sigma-Aldrich

Human M2 Macrophages (hMDM-MCSF(-))

Monocyte-derived, single donor, 1.5 million cryopreserved cells

别名:

hMDM-MCSF(-) cells

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About This Item

UNSPSC代码:
41106514
NACRES:
NA.81

生物来源

human blood (monocyte)

包装

pkg of 1,500,000 cells

形态学

(macrophages)

技术

cell culture | mammalian: suitable

运输

dry ice

储存温度

−196°C

一般描述

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

细胞系来源

Monocyte

应用

Cryopreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.Cryopreserved human macrophages are produced from monocytes in M2 Macrophage Generation Media DXF and are available as non-activated, fully qualified M2- (M-CSF) polarized cells. After 9-10 days of differentiation, the M2 macrophages are cryopreserved using Cryo-SFM. Each cryo vial contains more than 1.5 million viable cells after thawing.

警告

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

传代培养常规

Click here for more information.

其他说明

Recommended Plating Density: 200000 cells per cm2Tested Markers: CD163 positive, CD68 positiveNote: The plated cells do not proliferate. Recommended plating density for obtaining an adherent cell monolayer with 70-90% confluency.

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Recommended Primary Cell Culture Media:Link
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免责声明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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实验方案

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Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

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