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Merck
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主要文件

安全信息

C-12375

Sigma-Aldrich

Human Cardiac Fibroblasts (HCF)

500,000 cryopreserved cells

别名:

HCF cells

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About This Item

UNSPSC代码:
41106514
NACRES:
NA.81

生物来源

human heart (ventricles)

包装

pkg of 500,000 cells

形态学

(fibroblast)

技术

cell culture | mammalian: suitable

相关疾病

cardiovascular diseases

运输

dry ice

储存温度

−196°C

相关类别

一般描述

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

细胞系来源

Heart

应用

Primary Human Cardiac Fibroblasts (HCF) are isolated from the ventricles of the adult heart and are provided in a cryopreserved format. They play a central role in the maintenance of the extracellular matrix in the normal heart and the synthesis of growth factors and cytokines. Under pathophysiological conditions, cardiac fibroblasts are involved in scar formation after myocardial infarction, cardiac fibrosis, and cardiac hypertrophy. HCF can be used to study such processes in vitro.

质量

Rigid quality control tests are performed for each lot of Human Fibroblasts. They are tested for cell morphology, adherence rate and cell viability. Flow cytometric analyses of the fibroblast specific antigen CD90 are performed for each lot. Growth performance is tested through multiple passages up to 10 or 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

警告

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

传代培养常规

Click here for more information.

其他说明

Recommended Plating Density: 3500 - 7000 cells per cm2Passage After Thawing: P2Tested Markers: CD90 positive, Smooth muscle alpha-actin negative, slow muscle myosin negativeGuaranteed population doublings: >15

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免责声明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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