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生物来源
human dermis
包装
pkg of 500,000 cells
形态学
( dermal)
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
相关类别
一般描述
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
细胞系来源
Skin
应用
Primary Human Follicle Dermal Papilla Cells (HFDPC) are isolated mainly from human dermis of the scalp, originating from the occipital or temple region and are provided in a cryopreserved format. Information on donor hair and skin color is available on request. HFDPC stain positive for alkaline phosphatase.Dermal papillae are embedded in a laminin and collagen IV rich extracellular matrix at the base of the hair follicles. They are essential for the induction and maintenance of hair growth. Since they have androgen receptors, they can be used for in vitro screening of androgen blocking reagents.
质量
Rigid quality control tests are performed for each lot of Follicle Dermal Papilla Cells. They are tested for cell morphology, adherence rate, and cell viability. Immunohistochemical tests for significant markers, e.g. alkaline phosphatase, are carried out for each lot. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).
警告
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
传代培养常规
Click here for more information.
其他说明
Recommended Plating Density: 5000 - 10000 cells per cm2Passage After Thawing: P2Tested Markers: Alkaline phosphatase positiveGuaranteed population doublings: >10
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Recommended Primary Cell Culture Media:Link
免责声明
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
商品
Primary human follicle dermal papilla cells and optimized culture medium. Protocols for handling, cell passaging, media, and product use.
什么是原代细胞?关于动物和人类原代细胞系和培养基的全面概述,以及它们如何应用于生物医学细胞培养应用。相对于细胞系,原代培养物的均一性较低。
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