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主要文件

安全信息

C-12003

Sigma-Aldrich

Normal Human Epidermal Keratinocytes (NHEK)

Adult, single donor, 500,000 cryopreserved cells

别名:

NHEK cells

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20 PKG
¥935.90
200 PKG
¥9,325.80

¥935.90


预计发货时间2025年6月20日详情


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变更视图
20 PKG
¥935.90
200 PKG
¥9,325.80

About This Item

UNSPSC代码:
41106514
NACRES:
NA.81

¥935.90


预计发货时间2025年6月20日详情


获取大包装报价

生物来源

human epidermis

包装

pkg of 500,000 cells

形态学

( epidermal)

技术

cell culture | mammalian: suitable

运输

dry ice

储存温度

−196°C

一般描述

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

细胞系来源

Skin

应用

Primary Normal Human Epidermal Keratinocytes (NHEK) are isolated from the epidermis of adult skin (different locations) from a single donor and are provided in a cryopreserved format. They are the major cell type in the epidermis, making up about 90% of the cells.Epidermal keratinocytes originate in the stratum basale and move up through the layers of the epidermis. During this movement, they undergo gradual differentiation and morphology changes until they reach the stratum corneum, where they form a layer of nucleus-free, flat, and highly keratinized squamous cells. This layer forms an effective barrier to the entry of infectious agents into the body and minimizes moisture loss.Keratinocytes are also able to produce a variety of cytokines, growth factors, interleukins and complement factors. Therefore keratinocytes are important for wound healing, inflammation, and immune response.Normal Human Dermal Fibroblasts (NHDF) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available on request.

质量

Rigid quality control tests are performed for each lot of Normal Human Epidermal Keratinocytes. They are tested for cell morphology, adherence rate, and cell viability. Furthermore, immunohistochemical tests for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

警告

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

传代培养常规

Click here for more information.

其他说明

Recommended Plating Density: 5000 cells per cm2Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: >15

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Recommended Primary Cell Culture Media:Link

免责声明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

高风险级别生物产品--人源产品

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