form
lyophilized powder
Quality Level
specific activity
≥0.20 unit/mg solid
mol wt
>5 kDa
storage temp.
2-8°C
Application
从肺炎克雷伯氏菌中分离的柠檬酸裂解酶可用于消化低分子量人乳组分(>5 kDa,简称为 5kF)。
Biochem/physiol Actions
柠檬酸裂解酶存在于若干微生物中,用于催化柠檬酸盐裂解的第一步,形成草乙酸和乙酸盐。该酶含有 3 个多肽亚基,α-亚基(转移酶)、β-亚基(酰基裂解酶)和 γ-亚基(酰基-载体蛋白)。
Physical form
冻干品,含有牛血清白蛋白、蔗糖和 MgSO4
Other Notes
在 pH 7.6,25℃ 条件下,一个单位每分钟将 1.0 μmole 柠檬酸盐转化为草乙酸。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
常规特殊物品
此项目有
M Hupperich et al.
European journal of biochemistry, 192(1), 161-166 (1990-08-28)
The primary structure of the beta-subunit (acyl lyase subunit) of citrate lyase from Klebsiella pneumoniae (ATCC 13,882) was determined with protein chemical methods. The polypeptide chain consists of 289 amino acid residues and has a molecular mass of 31,352 Da.
Djamel Drider et al.
Genetics and molecular research : GMR, 3(2), 273-281 (2004-07-22)
Citrate is present in many natural substrates, such as milk, vegetables and fruits, and its metabolism by lactic acid bacteria (LAB) plays an important role in food fermentation. The industrial importance of LAB stems mainly from their ability to convert
M Martín et al.
FEMS microbiology letters, 174(2), 231-238 (1999-05-26)
The citMCDEFGRP cluster from Leuconostoc paramesenteroides involved in citrate utilization was cloned and its nucleotide sequence determined. Homology of the inferred gene products with characterized enzymes reveals that citP encodes the citrate permease P, citC the citrate ligase and citDEF
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| C0897-100UN | 04061835512256 |