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应用
从肺炎克雷伯氏菌中分离的柠檬酸裂解酶可用于消化低分子量人乳组分(>5 kDa,简称为 5kF)。
生化/生理作用
柠檬酸裂解酶存在于若干微生物中,用于催化柠檬酸盐裂解的第一步,形成草乙酸和乙酸盐。该酶含有 3 个多肽亚基,α-亚基(转移酶)、β-亚基(酰基裂解酶)和 γ-亚基(酰基-载体蛋白)。
单位定义
在 pH 7.6,25℃ 条件下,一个单位每分钟将 1.0 μmole 柠檬酸盐转化为草乙酸。
外形
冻干品,含有牛血清白蛋白、蔗糖和 MgSO4
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
含少量动物源组分生物产品
常规特殊物品
从最新的版本中选择一种:
分析证书(COA)
Archives of microbiology, 175(1), 75-78 (2001-03-29)
Hydrogenobacter thermophilus strain TK-6 was observed to grow anaerobically on nitrate as an electron acceptor when molecular hydrogen was used as an energy source. Nitrite was detected as the product of a respiratory reaction. 15NO, 15N2O, and 15N2 were detected
Isolation of subunits of citrate lyase and characterization of their function in the enzyme complex.
Proceedings of the National Academy of Sciences of the United States of America, 72(9), 3458-3462 (1975-09-01)
Citrate lyase [EC 4.1.3.6; citrate oxaloacetate-lyase (pro-3S-CH2-COO--leads to acetate)] from Klebsiella aerogenes has been dissociated with urea; the three different subunits, alpha-chain (molecular weight congruent to 54,000), beta-chain (molecular weight congruent to 32,000), and gamma-chain (acyl carrier protein; molecular weight
Isotopes in environmental and health studies, 40(3), 181-190 (2004-09-17)
The use of stable isotope techniques for the reconstruction of diets has increased over the last decade. However, isotopic ratios in an animal are not only affected by the composition of the feed, but also by the amount of feed
Citric acid mediates the iron absorption from low molecular weight human milk fractions
Journal of Agricultural and Food Chemistry, 61(46), 11151-11157 (2013)
Journal of bacteriology, 182(14), 3904-3912 (2000-06-27)
In this study we describe the expression pattern of the Leuconostoc paramesenteroides citMCDEFGRP operon in response to the addition of citrate to the growth medium. An 8.8-kb polycistronic transcript, which includes the citMCDEFGRP genes, was identified; its synthesis was dramatically
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