C0897
柠檬酸裂解酶 来源于肺炎克雷伯氏杆菌
lyophilized powder, ≥0.20 unit/mg solid
别名:
柠檬酸草酰乙酸裂解酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-740-7
MDL number:
Specific activity:
≥0.20 unit/mg solid
form
lyophilized powder
specific activity
≥0.20 unit/mg solid
mol wt
>5 kDa
storage temp.
2-8°C
Quality Level
Application
从肺炎克雷伯氏菌中分离的柠檬酸裂解酶可用于消化低分子量人乳组分（>5 kDa，简称为 5kF）。
Biochem/physiol Actions
柠檬酸裂解酶存在于若干微生物中，用于催化柠檬酸盐裂解的第一步，形成草乙酸和乙酸盐。该酶含有 3 个多肽亚基，α-亚基（转移酶）、β-亚基（酰基裂解酶）和 γ-亚基（酰基-载体蛋白）。
Physical form
冻干品，含有牛血清白蛋白、蔗糖和 MgSO4
Other Notes
在 pH 7.6，25℃ 条件下，一个单位每分钟将 1.0 μmole 柠檬酸盐转化为草乙酸。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
含少量动物源组分生物产品
常规特殊物品
此项目有
M Hupperich et al.
European journal of biochemistry, 192(1), 161-166 (1990-08-28)
The primary structure of the beta-subunit (acyl lyase subunit) of citrate lyase from Klebsiella pneumoniae (ATCC 13,882) was determined with protein chemical methods. The polypeptide chain consists of 289 amino acid residues and has a molecular mass of 31,352 Da.
Djamel Drider et al.
Genetics and molecular research : GMR, 3(2), 273-281 (2004-07-22)
Citrate is present in many natural substrates, such as milk, vegetables and fruits, and its metabolism by lactic acid bacteria (LAB) plays an important role in food fermentation. The industrial importance of LAB stems mainly from their ability to convert
M Martín et al.
FEMS microbiology letters, 174(2), 231-238 (1999-05-26)
The citMCDEFGRP cluster from Leuconostoc paramesenteroides involved in citrate utilization was cloned and its nucleotide sequence determined. Homology of the inferred gene products with characterized enzymes reveals that citP encodes the citrate permease P, citC the citrate ligase and citDEF
Isolation of subunits of citrate lyase and characterization of their function in the enzyme complex.
P Dimroth et al.
Proceedings of the National Academy of Sciences of the United States of America, 72(9), 3458-3462 (1975-09-01)
Citrate lyase [EC 4.1.3.6; citrate oxaloacetate-lyase (pro-3S-CH2-COO--leads to acetate)] from Klebsiella aerogenes has been dissociated with urea; the three different subunits, alpha-chain (molecular weight congruent to 54,000), beta-chain (molecular weight congruent to 32,000), and gamma-chain (acyl carrier protein; molecular weight
M Suzuki et al.
Archives of microbiology, 175(1), 75-78 (2001-03-29)
Hydrogenobacter thermophilus strain TK-6 was observed to grow anaerobically on nitrate as an electron acceptor when molecular hydrogen was used as an energy source. Nitrite was detected as the product of a respiratory reaction. 15NO, 15N2O, and 15N2 were detected
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