形式
glycerol solution
质量水平
比活
≥1500 units/mg protein
分子量
dimer subunit mol wt 70 kDa
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
sheep ... COX1(808251)
一般描述
环氧合酶 1 是一种分子量为 71 kDa 的膜结合蛋白，主要存在于内质网中。它有三个结构域，即表皮生长因子（EGF）样域、酶结构域和膜结合域。环氧合酶 1 介导前列腺素合成，并受抗炎非甾体药物调节。
应用
在骨关节炎样品的蛋白免疫印迹分析 和前列腺素合成酶活性测定中，来自绵羊的环氧合酶 1 已用作阳性对照蛋白。
生化/生理作用
COX-1 催化花生四烯酸转化为前列腺素 H 2 （前列腺素、血栓素和前列环素生物合成的第一步）。它参与类花生酸的稳态角色，并在几乎所有动物组织中组成性表达。其中，花生四烯酸的表观 K M 为 8.3μM 。
单位定义
在37°C下，一个单位在含 100μM花生四烯酸盐、5 mM EDTA、2 mM 苯酚和 1 μM血色素的 0.1 MTris-HCl 缓冲液 (pH 8) 中每分钟消耗 1 纳摩尔氧气。
外形
溶液溶于 80 mM Tris-HCl，pH 8，含 0.1% 吐温 ® 20，300μM 二乙基二硫代氨基甲酸酯和 10% 甘油。
法律信息
TWEEN is a registered trademark of Croda International PLC
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
Cyclo-oxygenase isoenzymes: physiological and pharmacological role
Kam PCA and See AUL
Anaesthesia, 55(5), 442-449 (2000)
Maurizio Anzini et al.
Journal of medicinal chemistry, 56(8), 3191-3206 (2013-03-29)
A series of 3-substituted 1,5-diarylpyrroles bearing a nitrooxyalkyl side chain linked to different spacers were designed. New classes of pyrrole-derived nitrooxyalkyl inverse esters, carbonates, and ethers (7-10) as COX-2 selective inhibitors and NO donors were synthesized and are herein reported.
S Ohki et al.
The Journal of biological chemistry, 254(3), 829-836 (1979-02-10)
The highly purified prostaglandin endoperoxide synthetase from bovine vesicular gland microsomes had two still unresolved enzyme activities; the oxygenative cyclization of 8,11,14-eicosatrienoic acid to produce prostaglandin G1 and the conversion of the 15-hydro-peroxide of prostaglandin G1 to a 15-hydroxyl group
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Diseases due to cigarette smoke exposure, including chronic obstructive pulmonary disease (COPD) and lung cancer, are associated with chronic inflammation typified by the increased expression of cyclooxygenase-2 (COX-2) protein. RelB is an NF-κB family member that suppresses cigarette smoke induction
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