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物料
dextran beads
无菌性
non-sterile
粒径
60-87 μm
密度
1.03 g/cm3 at 25 °C
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一般描述
A general-purpose microcarrier for most established, anchorage-dependent cell lines.
High yield culture of anchorage-dependent cells: Microcarrier culture is a technique which makes possible the practical high yield culture of anchorage-dependent cells. Cytodex has been specifically developed for the culture of a wide range of animal cells in culture volumes ranging from a few mL to more than 6000 L. Using Cytodex in simple suspension culture systems provides yields of several million cells per mL.
Suitable for most established cell lines: Formed by substituting a cross-linked dextran matrix with positively charged DEAE* groups distributed throughout the matrix, is a general purpose microcarrier. It is particularly suitable for most established cell lines and for production of viruses or cell products from cultures of primary cells and normal diploid cell strains.
High yield culture of anchorage-dependent cells: Microcarrier culture is a technique which makes possible the practical high yield culture of anchorage-dependent cells. Cytodex has been specifically developed for the culture of a wide range of animal cells in culture volumes ranging from a few mL to more than 6000 L. Using Cytodex in simple suspension culture systems provides yields of several million cells per mL.
Suitable for most established cell lines: Formed by substituting a cross-linked dextran matrix with positively charged DEAE* groups distributed throughout the matrix, is a general purpose microcarrier. It is particularly suitable for most established cell lines and for production of viruses or cell products from cultures of primary cells and normal diploid cell strains.
应用
用于一般微载体培养。
本品溶胀于液体溶液时（如使用），平均粒径为 190 μm。60-87 μm 的粒度与干燥形式的微珠有关。
本品溶胀于液体溶液时（如使用），平均粒径为 190 μm。60-87 μm 的粒度与干燥形式的微珠有关。
特点和优势
- General-purpose microcarrier for most established, anchorage-dependent cell lines.
- Contains positive charges throughout the microporous matrix.
- Transparent for easy microscopic examination of attached cells.
其他说明
含正电荷 DEAE 基团的葡聚糖珠。
制备说明
使用前，将微球溶于 PBS (50 mL/g) 中，并在 120°C 下高压灭菌。
法律信息
Cytodex is a registered trademark of Cytiva
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
C Hagen et al.
Journal of microscopy, 230(Pt 2), 288-296 (2008-05-01)
A method is described employing microcarrier spheres of cross-linked dextran for obtaining ultra- and semithin vitreous sections from high-pressure frozen anchorage-dependent (mammalian) cells. Avoiding trypsination or scraping cells off from the culture surface, the presented approach allows for cryoimmobilization, cryosectioning
S Mehdi Hassanzadeh et al.
Pakistan journal of biological sciences : PJBS, 14(7), 441-448 (2011-09-10)
To achieve higher titer of rabies virus higher density of host cells will need. In this study, capability of FibraCel disks packed in 500 mL spinner basket versus Cytodex-1 in 500 mL spinner flask was investigated for propagation of Vero
Pierre Danhier et al.
PloS one, 8(1), e53324-e53324 (2013-02-06)
Cell detachment is a procedure routinely performed in cell culture and a necessary step in many biochemical assays including the determination of oxygen consumption rates (OCR) in vitro. In vivo, cell detachment has been shown to exert profound metabolic influences
Khaled Trabelsi et al.
Applied microbiology and biotechnology, 93(3), 1031-1040 (2011-09-22)
Measles vaccination remains the most efficient way to control the spread of the virus. This work focuses on the production of a measles vaccine using stirred conditions as an advanced option for process scale up. Non-porous Cytodex 1 microcarriers were
Quanyu Zhao et al.
Biotechnology progress, 19(5), 1569-1573 (2003-10-04)
Toward the development of an in vitro cultivation of marine sponge cells for sustainable production of bioactive metabolites, the attachment characteristics of marine sponge cells of Hymeniacidon perleve on three types of microcarriers, Hillex, Cytodex 3, and glass beads, were
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