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质量水平
无菌性
γ-irradiated
类型
Type IV-S
产品线
BioReagent
表单
lyophilized powder
技术
cell culture | mammalian: suitable
杂质
salt, essentially free
溶解性
PBS: 5 mg/mL, slightly hazy
H2O: soluble, slightly hazy
储存温度
−20°C
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应用
来自洋刀豆(刀豆)的伴刀豆球蛋白A已用于以下情形:
- 诱导脾胚芽生殖
- 从B6和lpr脾细胞生成ConA blast靶标
- 刺激雌激素和安慰剂治疗小鼠的脾淋巴细胞
生化/生理作用
刀豆素 A 无血型特异性,但对末端 α-D-甘露糖基和 α-D-葡糖基残基具有亲和力。需要 Ca2+ 和 Mn2+ 离子存在才能显示活性。刀豆素 A 在 pH 6.5 或更低的条件下解离为二聚体。pH 在 5.8 至 7.0 之间时,刀豆素 A 以四聚体形式存在;pH 高于 7.0 时,形成更高的聚合体。根据聚合程度不同,刀豆素 A 可能显示出具有促有丝分裂的活性。琥珀酰化可以生成一种能够在 pH 高于 5.6 时仍保持二聚体形式的活性二聚体。
分析说明
据报道,凝集活性以μg/ml表示,并且在Ca2+ 和Mn2+ 浓度为1 mg/ml、pH 6.8的磷酸缓冲盐溶液中进行连续稀释测定。该活性是在25°C下孵育1小时的2%人红细胞悬浮液可以凝集的最低浓度。
警示用语:
Danger
危险声明
危险分类
Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
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