一般描述
氯过氧化物酶是由真菌分泌的一种含血红素的糖蛋白。氯过氧化物酶(CPO)是一种以高铁血红素IX为辅基的胞外血红素糖酶。
应用
它已被用于研究聚合物纳米反应器中的生物催化氧化。
氯过氧化物酶（来自Caldariomyces fumagois）在131I标记和蛋白质溴化和长期分离程序的大分子36Cl标记中，可作为乳过氧化物酶的有效替代品。 它已用于研究聚合物纳米反应器中的生物催化氧化。
在 131 I 离子标记研究、蛋白质溴化和 36 Cl 标记大分子的长期分离程序中，可作为乳过氧化物酶的有效替代品。
生化/生理作用
氯过氧化物酶(CPO)由真菌分泌产生，具有广谱的化学反应性。它是一种依赖过氧化物的氯化酶，也催化过氧化物酶的脱氢、过氧化氢酶的过氧化氢(H2O2)降解和细胞色素P450的氧原子插入反应。该酶具有类似于细胞色素P450的磁性和光谱特性。来源于Caldariomyces fumago的氯过氧化物酶可氯化多环芳烃(PAH)等芳香烃类化合物。氯过氧化物酶具有潜在健康风险，因其致癌和致突变性而又在环境中广泛散布。
单位定义
25°C、pH 2.75、存在氯化钾和 H2O2 时，1 个单位每分钟将催化 1.0 μmol 的单氯二甲基酮转化为二氯二甲基酮。
外形
0.1 M 磷酸钠中的粗混悬液，pH 值约为 4.5
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
C M Hosten et al.
The Journal of biological chemistry, 269(19), 13966-13978 (1994-05-13)
Near-ultraviolet resonance Raman spectra of chloroperoxidase derivatives and high valent intermediates show frequencies that can be systematically assigned. In accord with previous observations of low v4 frequencies for the ferric enzyme, and quite low v4 frequencies for the ferrous enzyme
R Vázquez-Duhalt et al.
Phytochemistry, 58(6), 929-933 (2001-10-31)
Chloroperoxidase from Caldariomyces fumago was able to chlorinate 17 of 20 aromatic hydrocarbons assayed in the presence of hydrogen peroxide and chloride ions. Reaction rates varied from 0.6 min(-1) for naphthalene to 758 min(-1) for 9-methylanthracene. Mono-, di- and tri-chlorinated
Ilona F Persoon et al.
Journal of endodontics, 38(1), 72-74 (2011-12-14)
The aim of this study was to explore the antimicrobial effect of vanadium chloroperoxidase (VCPO) reaction products on Enterococcus faecalis biofilms of 4 different strains. Twenty-four-hour biofilms of E. faecalis strains V583, ER5/1, E2, and OS-16 were incubated in mixtures
Alexander N Morozov et al.
Biophysical journal, 100(4), 1066-1075 (2011-02-16)
Molecular dynamics simulations of an explicitly solvated cis-β-methylstyrene/chloroperoxidase-Compound I complex are performed to determine the cause of the high enantiospecificity of epoxidation. From the simulations, a two-dimensional free energy potential is calculated to distinguish binding potential wells from which reaction
Adam C Chamberlin et al.
The journal of physical chemistry. B, 115(13), 3642-3647 (2011-03-18)
OLYP/TZP calculations on two symmetrized model complexes [Fe(TPP)(py)(2)](2+) and [Fe(TPP)(PhNC)(2)](2+) (TPP = meso-tetraphenylporphyrin, py = pyridine, PhNC = phenylisocyanide) reveal dense manifolds of low-energy electronic states. For the latter complex, broken-symmetry calculations successfully reproduce the unique S = 0 ground
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门