荧光
λex 496 nm; λem 516 nm
运输
wet ice
储存温度
2-8°C
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一般描述
Baseclick EdU Flow Cytometry Kit为直接检测DNA合成提供了替代BrdU和[3H]thymidine检测的出色方法。EdU（5-乙炔基-2'.-脱氧尿苷）是一种胸苷核苷类似物，在活性DNA合成期间掺入到DNA之中。与BrdU检测法不同，EdU Flow Cytometry Assay不依赖抗体，因而不需进行DNA变性来检测掺入的核苷。恰恰相反，EdU Flow Cytometry Kit利用点击化学法来检测不同种类的染料荧光示值。此外，该试剂盒的实验方案经过简化，减少了总步骤数同时大幅缩减了总耗时。简单的点击化学法检测步骤可在30分钟内完成，在结果的内容和情境丰富程度方面，堪比多重分析方法。
应用
流式细胞术
警示用语：
Danger
危险分类
Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 1B - Repr. 2 - Skin Sens. 1
储存分类代码
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Abdul S Qadir et al.
iScience, 24(11), 103348-103348 (2021-11-25)
The apoptosis inducing receptor CD95/Fas has multiple tumorigenic activities. In different genetically engineered mouse models tumor-expressed CD95 was shown to be critical for cell growth. Using a combination of immune-deficient and immune-competent mouse models, we now establish that loss of
Patrick J Metz et al.
Cell reports, 30(6), 1935-1950 (2020-02-13)
Alternative splicing is well understood to enhance proteome diversity as cells respond to stimuli. However, mechanistic understanding for how the spliceosome processes precursor messenger RNA (mRNA) transcripts to achieve template diversification is incomplete. We use recently developed enzymatic inhibitors of
Yongmao Yu et al.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
Fatemah Chehrehasa et al.
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a
相关内容
Experimental studies measuring cell proliferation have had implications in cancer biology, immunology, cell biology, and developmental biology.
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