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荧光
λex 584 nm; λem 603 nm
运输
wet ice
储存温度
2-8°C
一般描述
EdU(5-乙炔基-2′-脱氧尿苷)是一种胸腺嘧啶核苷类似物，可在DNA合成活跃期掺入到DNA中。与溴脱氧尿苷(BrdU)检测法不同，EdU点击检测法(EdU-Click Assay)不是基于抗体，因此并不需要DNA变性以检测掺入的核苷。EdU点击检测法采用的是点击化学(click chemistry)技术，通过获取各种染料的荧光信号进行检测。此外，简化的检测方案不仅减少总步骤数，而且大幅缩短总用时。这一简单的点击化学检测程序在30分钟内即可完成，兼容的多重检测可提供内容和含义丰富的结果。
应用
成像
警示用语：
Danger
危险分类
Aquatic Chronic 3 - Eye Irrit. 2 - Muta. 1B - Repr. 2
WGK
WGK 3
Yongmao Yu et al.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
Fatemah Chehrehasa et al.
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a
相关内容
Experimental studies measuring cell proliferation have had implications in cancer biology, immunology, cell biology, and developmental biology.
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