荧光
λex 496 nm; λem 516 nm
运输
wet ice
储存温度
2-8°C
一般描述
EdU(5-乙炔基-2′-脱氧尿苷)是一种胸腺嘧啶核苷类似物，可在DNA合成活跃期掺入到DNA中。与溴脱氧尿苷(BrdU)检测法不同，EdU点击(EdU-Click)检测法不是基于抗体，因此并不需要DNA变性以检测掺入的核苷。EdU点击检测法采用的是点击化学(click chemistry)技术，通过获取各种染料的荧光信号进行检测。此外，简化的检测方案不仅减少总步骤数，而且大幅缩短总用时。这一简单的点击化学检测程序在30分钟内即可完成，兼容的多重检测可提供内容和含义丰富的结果。
应用
成像
警示用语：
Danger
危险分类
Aquatic Chronic 3 - Eye Irrit. 2 - Muta. 1B - Repr. 2
储存分类代码
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Drake S Edwards et al.
Cell reports, 32(12), 108166-108166 (2020-09-24)
Effective spatio-temporal control of transcription and replication during S-phase is paramount to maintaining genomic integrity and cell survival. Dysregulation of these systems can lead to conflicts between the transcription and replication machinery, causing DNA damage and cell death. BRD4 allows
Federico Ghiselli et al.
BMC molecular and cell biology, 22(1), 12-12 (2021-02-14)
Enterocytes exert an absorptive and protective function in the intestine, and they encounter many different challenging factors such as feed, bacteria, and parasites. An intestinal epithelial in vitro model can help to understand how enterocytes are affected by these factors
Yongmao Yu et al.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
Fatemah Chehrehasa et al.
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a
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Experimental studies measuring cell proliferation have had implications in cancer biology, immunology, cell biology, and developmental biology.
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