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荧光
λex 496 nm; λem 516 nm
运输
wet ice
储存温度
2-8°C
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一般描述
Baseclick EdU In Vivo Kit为全部动物的细胞增殖检测提供了替代BrdU 及[[3H]thymidine检测的出色方法，且未显示出任何的毒性效果。EdU（5-乙炔基-2'.-脱氧尿苷）是一种胸苷核苷类似物，在活性DNA合成期间掺入到DNA之中。与BrdU检测法不同，EdU In Vivo Assay不依赖抗体，因而不需进行DNA变性来检测掺入的核苷。恰恰相反，EdU In Vivo Kit利用点击化学法来检测不同种类的染料荧光示值。此外，该试剂盒的实验方案经过简化，减少了总步骤数同时大幅缩减了总耗时。简单的点击化学法检测步骤可在30分钟内完成，在结果的内容和情境丰富程度方面，堪比多重分析法。这一款体内试剂盒的设计秉承一种理念，即使您可以结合如下三种baseclick EdU细胞增殖试剂盒之中的一种：
1. 成像试剂盒 (BCK-IV-IM)
2. 流式细胞术试剂盒(BCK-IV-FC)
3. 高通量筛选 – HTS试剂盒 (BCK-IV-HTS)
您可以针对自己的动物模型选择上述试剂盒及自选的染料和合适的EdU含量。
根据您的动物模型或需要检测的动物数量，您可以选择S、M和L三种规格的试剂盒，这三种规格的EdU含量依次递增，如下表所示。
试剂盒规格 EdU含量
S 50 mg
M 500 mg
L 1000 mg
1. 成像试剂盒 (BCK-IV-IM)
2. 流式细胞术试剂盒(BCK-IV-FC)
3. 高通量筛选 – HTS试剂盒 (BCK-IV-HTS)
您可以针对自己的动物模型选择上述试剂盒及自选的染料和合适的EdU含量。
根据您的动物模型或需要检测的动物数量，您可以选择S、M和L三种规格的试剂盒，这三种规格的EdU含量依次递增，如下表所示。
试剂盒规格 EdU含量
S 50 mg
M 500 mg
L 1000 mg
应用
体内，成像
警示用语：
Danger
危险分类
Aquatic Chronic 3 - Eye Irrit. 2 - Muta. 1B - Repr. 2
WGK
WGK 3
Junhui Zhang et al.
Frontiers in pharmacology, 10, 368-368 (2019-05-02)
Endothelial cells play a critical role in the process of angiogenesis during skin wound healing. The migration and proliferation of endothelial cells are processes that are initiated by the hypoxic microenvironment in a wound, but the underlying mechanisms remain largely
Junhui Zhang et al.
International journal of biological sciences, 15(9), 1962-1976 (2019-09-17)
Both cell migration and proliferation are indispensable parts of reepithelialization during skin wound healing, which is a complex process for which the underlying molecular mechanisms are largely unknown. Here, we identify a novel role for microtubule-associated protein 4 (MAP4), a
Lingfei Li et al.
Cell communication and signaling : CCS, 20(1), 115-115 (2022-07-29)
Diabetic nephropathy (DN) involves various structural and functional changes because of chronic glycemic assault and kidney failure. Proteinuria is an early clinical manifestation of DN, but the associated pathogenesis remains elusive. This study aimed to investigate the role of microtubule
Yongmao Yu et al.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
Fatemah Chehrehasa et al.
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a
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