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Merck
CN
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文件

B9754

Sigma-Aldrich

2-二(2-羟乙基)氨基-2-羟甲基-1,3-丙二醇

≥98.0% (titration)

别名:

2-二(2-羟乙基)氨基-2-羟甲基-1,3-丙二醇, 2-双(羟基甲基)-2,2′,2″-次氮基三乙醇, 双(2-羟乙基)氨基-三(羟甲基)甲烷

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About This Item

经验公式（希尔记法）:
C8H19NO5
CAS号:
6976-37-0
分子量:
209.24
Beilstein:
2205275
EC 号:
230-237-7
MDL编号:
MFCD00002853
UNSPSC代码:
12161700
PubChem化学物质编号:
24892155
NACRES:
NA.25

质量水平

400

检测方案

≥98.0% (titration)

形式

crystalline powder

储存条件

dry at room temperature

技术

UV/Vis spectroscopy: suitable
electrophoresis: suitable

颜色

white

有效pH范围

5.8-7.2

pKa (25 °C)

6.5

溶解性

H2O: 500 mg/mL, clear, colorless to very faintly yellow

适用性

suitable for Western blot
suitable for column chromatography of proteins

应用

diagnostic assay manufacturing
general analytical
microbiology

SMILES字符串

OCCN(CCO)C(CO)(CO)CO

InChI

1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2

InChI key

OWMVSZAMULFTJU-UHFFFAOYSA-N

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相关类别

一般描述

Bis-tris 甲烷，也称为 BIS-TRIS 或 BTM，是一种广泛应用于生物化学和生物研究的两性离子缓冲剂。它在 pH 值范围为 5.8 至 7.2 的水溶液中有效发挥作用，并且其解离常数变化最小。此外，它可以用作剧毒缓冲剂二甲胂酸盐的替代品，并且还因其与不同金属形成络合物的能力而受到认可。

应用

Bis-Tris已被使用：
  • 作为电泳缓冲液
  • 作为蛋白质印迹转移缓冲液的组分

特点和优势

  • 适用于生物和生化研究
  • 在 pH 5.8-7.2 (25 °C) 范围内有效缓冲，pKa 为 6.5 (25 °C)

其他说明

如需了解生化试剂系列的更多信息，请填写此表。

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H319

危险分类

Eye Irrit. 2

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

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实验方案

Enzymatic Activity of Glucose-6-Phosphatase [EC 3.1.3.9]

To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.

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