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Merck
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安全信息

B9655

Sigma-Aldrich

Anti-Avidin antibody, Mouse monoclonal, biotin conjugate

clone WC19.10, purified from hybridoma cell culture

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别名:
Monoclonal Anti-Avidin
UNSPSC代码:
12352203
NACRES:
NA.46

生物来源

mouse

质量水平

偶联物

biotin conjugate

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

WC19.10, monoclonal

形式

buffered aqueous solution

技术

direct ELISA: suitable
dot blot: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

同位素/亚型

IgG1

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

相关类别

一般描述

Avidin is a glycoprotein (68 kDa) composed of four identical polypeptides chains isolated from egg whites.Monoclonal Anti-Avidin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
The antibody binds to the avidin molecule without interfering with the biotin-binding reaction. The product reacts specifically with egg white avidin, ExtrAvidin and their complexes formed with biotin. The antibody shows no cross-reaction with streptavidin.

免疫原

Purified egg white avidin.

应用

Anti-Avidin-Biotin antibody, Mouse monoclonal has been used for fluorescence in situ hybridization (FISH) analysis of chromosomes X and 8 in lymphocytes isolated from women

生化/生理作用

Avidin has a high binding affinity for the vitamin, biotin. Biotin conjugated monoclonal anti-avidin increases specific avidin-biotin complex promoting the sensitivity of avidin-biotin immunoassays.

外形

Solution supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

含少量动物源组分生物产品
常规特殊物品

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Murine monoclonal anti-avidin antibodies enhance the sensitivity of avidin-biotin immunoassays and immunohistologic staining
Cassano WF, et al.
Journal of Immunological Methods, 117(2), 169-174 (1989)
Chapter 16 - Biotechnological Approaches for Insect Pest Management
Integrated Pest Management Current Concepts and Ecological Perspective, 311-335 (2014)
Chapter 11 - (Strept)avidin?Biotin Systems
Bioconjugate Techniques, 465-505 (2013)
Jaeho Kim et al.
Ultrasonography (Seoul, Korea), 41(2), 382-393 (2021-12-29)
The purpose of this study was to evaluate if transcutaneous application of low-intensity ultrasound can locally enhance the effects of finasteride on hair growth in a murine model of androgenic alopecia (AA). AA mice (injected twice per week with testosterone
Spontaneous and spindle poison-induced micronuclei and chromosome non-disjunction in cytokinesis-blocked lymphocytes from two age groups of women
Bakou K, et al.
Mutagenesis, 17(3), 233-239 (2002)

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