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Merck
CN

B9410

BIS-TRIS丙烷

BioXtra, ≥99.0% (titration)

别名:

1,3-双[三(羟甲基)甲氨基]丙烷

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关于此项目

线性分子式:
CH2[CH2NHC(CH2OH)3]2
化学文摘社编号:
64431-96-5
分子量:
282.33
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
24892138
EC Number:
264-899-3
Beilstein/REAXYS Number:
1786109
MDL number:
MFCD00004689

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产品名称

BIS-TRIS丙烷, BioXtra, ≥99.0% (titration)

InChI key

HHKZCCWKTZRCCL-UHFFFAOYSA-N

InChI

1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2

SMILES string

OCC(CO)(CO)NCCCNC(CO)(CO)CO

product line

BioXtra

assay

≥99.0% (titration)

form

powder

impurities

≤0.005% Phosphorus (P)
≤0.1% Insoluble matter

ign. residue

≤0.1%

pH

10.0-12.0 (1 M in H2O)

useful pH range

6.3-9.5

pKa (25 °C)

(1) 6.8, (2) 9.0

mp

164-165 °C (lit.)

solubility

H2O: 1 M, clear, colorless

anion traces

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.005%
Pb: ≤0.001%
Zn: ≤0.0005%

absorption

≤0.05 at 280 in H2O at 1 M
≤0.1 at 260 in H2O at 1 M

application(s)

diagnostic assay manufacturing

storage temp.

room temp

Quality Level

400

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相关类别

Application

  • 用于评估人类全血中全基因组DNA甲基化的毛细管电泳紫外检测方法: 在毛细管电泳法中，研究人员使用BIS-TRIS丙烷为缓冲剂来评估人类全血中的全DNA甲基化水平。缓冲液′对于维持电泳条件的稳定性和可重复性起到关键作用（Zinellu et al., 2019）。

  • 作为原始不产氧细菌高效电子供体的低电位锰离子: 在研究低电位锰离子作为细菌电子受体的作用中，采用BIS-TRIS丙烷对实验条件进行缓冲。 这些发现增进了我们对微生物系统中电子转移系统的理解（Deshmukh et al., 2018）。

  • 通过毛细管电泳紫外检测评估人类全血中全基因组DNA甲基化。: 该研究在毛细管电泳法中采用BIS-TRIS丙烷来评估DNA甲基化水平。该缓冲液′的性质确保了准确可靠的检测，这一点对表观遗传学研究很关键（Zinellu et al., 2017）。

Other Notes

很容易将 BIS-TRIS 丙烷产品规格与 BIS-TRIS 丙烷规格表进行比较。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000503827
0000503827
0000456836
0000456837
0000456836

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Hamed Tahmasbi et al.
Journal of pharmaceutical and biomedical analysis, 171, 1-7 (2019-04-07)
Secreted recombinant activated clotting factor VII activated (rFVIIa) in cell culture media missing gamma-carboxyglutamic acid (Gla) domain as a result of failure in gamma-carboxylation or cell lysis is called Gla-domainless impurity which has less negative charge compared to native rFVIIa.
Sami J O Varjo et al.
Journal of chromatography. A, 1081(1), 92-98 (2005-07-15)
Greater stability of liposome coatings and improved resolution of model steroids in capillary electrochromatography (CEC) were sought by adding small diamines (ethylenediamine, diaminopropane, bis-tris-propane, or N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid, HEPES)) to the liposome solution before coating of fused silica capillaries. The phospholipid
Joseph S Brunzelle et al.
Archives of biochemistry and biophysics, 474(1), 157-166 (2008-04-01)
The three-dimensional structure of the catalytically efficient beta-xylosidase from Selenomonas ruminantium in complex with competitive inhibitor 1,3-bis[tris(hydroxymethyl)methylamino]propane (BTP) was determined by using X-ray crystallography (1.3A resolution). Most H bonds between inhibitor and protein occur within subsite -1, including one between
P Gómez-Tagle et al.
Inorganic chemistry, 40(15), 3786-3796 (2001-07-10)
Potentiometric titrations of the mixtures of lanthanide(III) perchlorates and bis-Tris propane (BTP) reveal formation of dinuclear hydroxo complexes M2(BTP)2(OH)n(6-n), where M = La(III), Pr(III), Nd(III), Eu(III), Gd(III), and Dy(III) and n = 2, 4, 5, or 6, in the pH
Sarita V Mello et al.
Biomacromolecules, 4(4), 968-973 (2003-07-15)
In this paper, we describe the preparation and characterization of Langmuir and Langmuir-Blodgett (LB) monolayers of the enzyme organophosphorus acid anhydrolase (OPAA). Langmuir films of OPAA were characterized on different subphases, such as phosphate, ammonium carbonate, and bis-tris-propane buffers. Monolayers
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Product Information Sheet - B9410

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