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Merck
CN

B9183

Sigma-Aldrich

单克隆抗-HA

clone HA-7, purified from hybridoma cell culture

别名:

抗-HA, 抗-流感血凝素

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About This Item

MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.56

生物来源

mouse

质量水平

偶联物

biotin conjugate

抗体形式

purified from hybridoma cell culture

抗体产品类型

primary antibodies

克隆

HA-7, monoclonal

表单

buffered aqueous solution

浓度

~1 mg/mL

技术

western blot (chemiluminescent): 0.25-0.50 μg/mL using HA tagged fusion proteins in transiently transfected mammalian cell extracts.

运输

dry ice

储存温度

−20°C
2-8°C

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一般描述

The Monoclonal Anti-HA Biotin Conjugate antibody recognizes the HA tag sequence on the HA tagged fusion proteins when expressed at the N or C terminal to the fusion protein. This antibody is derived from the HA-7 hybridoma, which is produced from the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the amino acid residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH. Ascites fluid collected from the HA-7 hybridoma is then conjugated to biotin.

特异性

该抗体可识别HA-标签蛋白的天然和还原变性形式,并且可与 大肠杆菌 或哺乳动物表达的N-末端或C-末端HA-标签融合蛋白发生反应。

免疫原

与人流感病毒血凝素(HA)片段相对应的合成肽,称为HA-标签,与KLH结合

应用

Antibody suitable for immunoblotting.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

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储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品
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分析证书(COA)

Lot/Batch Number

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?

    Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Tragoolpua,K et al.
Biotechnology, 8(5) (2008)
J Zhang et al.
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High-risk, cancer-causing human papillomaviruses (HPV) cause infections of the epidermis that may progress to cancer, including cervical cancer. Viral persistence, contributed to by viral evasion of the host immune response, is associated with the likelihood of cancer developing. Langerhans cells
Emeline Ragonnaud et al.
Journal of immunotherapy (Hagerstown, Md. : 1997), 40(2), 51-61 (2017-02-07)
Currently available prophylactic vaccines have no therapeutic efficacy for preexisting human papillomavirus (HPVs) infections, do not target all oncogenic HPVs and are insufficient to eliminate the burden of HPV induced cancer. We aim to develop an alternative HPV vaccine which
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The Journal of clinical endocrinology and metabolism, 106(9), e3346-e3363 (2021-06-02)
Mechanisms underlying pituitary corticotroph adenoma adrenocorticotropin (ACTH) production are poorly understood, yet circulating ACTH levels closely correlate with adenoma phenotype and clinical outcomes. We characterized the 5' ends of proopiomelanocortin (POMC) gene transcripts, which encode the precursor polypeptide for ACTH
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eLife, 6 (2017-08-23)
Mutations in the human

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