form
liquid
concentration
1% in 3% acetic acid, pH 2.5
technique(s)
microbe id | staining: suitable
color
dark blue to very dark blue
pH range
2.4-2.6
Amax
0.45-0.60 (1:400 in H2O)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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Application
阿尔新蓝溶液已用于对羧化或硫酸化的酸性糖胺聚糖（GAG；粘多糖）进行组织学染色。在pH为2.5时，硫酸糖胺聚糖似乎是阿尔新蓝的首选底物。阿尔新蓝用于染色透明质酸（HA，一种非硫酸化糖胺聚糖），但透明质酸的染色强度受所用组织固定方法的影响。组织中糖胺聚糖的阿尔新蓝染色，可以通过添加诸如氯化镁的电解质来改进。
Biochem/physiol Actions
阿尔新蓝是一种阳离子染料。它与酸性糖胺聚糖形成不溶性复合物，从而有助于糖胺聚糖的定量测定。它还用于骨和软骨的染色。
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 45(8), 1157-1163 (1997-08-01)
The apparent intensity of hyaluronan (HA) staining in tissue sections can vary as a function of fixation techniques. We examined the histochemical distribution of HA in normal human skin using an HA-specific binding peptide derived from bovine nasal cartilage. The
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Recombinant platelet-derived growth factor (PDGF) and transforming growth factor beta 1 (TGF-beta 1) influence the rate of extracellular matrix formed in treated incisional wounds. Because incisional healing processes are difficult to quantify, a full-thickness excisional wound model in the rabbit
Differential staining of acid glycosaminoglycans (mucopolysaccharides) by alcian blue in salt solutions.
J E Scott et al.
Histochemie. Histochemistry. Histochimie, 5(3), 221-233 (1965-10-01)
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