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表单
liquid
质量水平
浓度
1% in 3% acetic acid, pH 2.5
技术
microbe id | staining: suitable
颜色
dark blue to very dark blue
pH值范围
2.4-2.6
Amax
0.45-0.60 (1:400 in H2O)
应用
diagnostic assay manufacturing
hematology
histology
储存温度
room temp
应用
阿尔新蓝溶液已用于对羧化或硫酸化的酸性糖胺聚糖(GAG;粘多糖)进行组织学染色。在pH为2.5时,硫酸糖胺聚糖似乎是阿尔新蓝的首选底物。阿尔新蓝用于染色透明质酸(HA,一种非硫酸化糖胺聚糖),但透明质酸的染色强度受所用组织固定方法的影响。组织中糖胺聚糖的阿尔新蓝染色,可以通过添加诸如氯化镁的电解质来改进。
生化/生理作用
阿尔新蓝是一种阳离子染料。它与酸性糖胺聚糖形成不溶性复合物,从而有助于糖胺聚糖的定量测定。它还用于骨和软骨的染色。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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The American journal of pathology, 138(3), 629-646 (1991-03-01)
Recombinant platelet-derived growth factor (PDGF) and transforming growth factor beta 1 (TGF-beta 1) influence the rate of extracellular matrix formed in treated incisional wounds. Because incisional healing processes are difficult to quantify, a full-thickness excisional wound model in the rabbit
Differential staining of acid glycosaminoglycans (mucopolysaccharides) by alcian blue in salt solutions.
J E Scott et al.
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A procedure for differential staining of cartilage and bone in whole formalin-fixed vertebrates.
Wassersug R J.
Stain Technology, 51(2), 131-134 (1976)
W Lin et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 45(8), 1157-1163 (1997-08-01)
The apparent intensity of hyaluronan (HA) staining in tissue sections can vary as a function of fixation techniques. We examined the histochemical distribution of HA in normal human skin using an HA-specific binding peptide derived from bovine nasal cartilage. The
The quantitative determination of glycosaminoglycans in urine with Alcian Blue 8GX.
Whiteman P.
The Biochemical Journal, 131(2), 351-357 (1973)
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