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CN
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文件

B7632

Sigma-Aldrich

N-苯甲酰基-Phe-Val-Arg-对硝基苯胺 盐酸盐

protease substrate

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别名:
N-苯甲酰基-L-苯丙氨酰-L-缬氨酰-L-精氨酸-4-硝基苯胺 盐酸盐
经验公式（希尔记法）:
C33H40N8O6 · HCl
CAS号:
54799-93-8
分子量:
681.18
Beilstein:
3027332
MDL编号:
MFCD00038397
UNSPSC代码:
12352204
PubChem化学物质编号:
24892011
NACRES:
NA.32

质量水平

200

检测方案

≥98% (TLC)

形式

powder

溶解性

methanol: 20 mg/mL, clear, colorless to light yellow

储存温度

−20°C

SMILES字符串

Cl.CC(C)[C@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)c2ccccc2)C(=O)N[C@@H](CCCNC(N)=N)C(=O)Nc3ccc(cc3)[N+]([O-])=O

InChI

1S/C33H40N8O6.ClH/c1-21(2)28(40-31(44)27(20-22-10-5-3-6-11-22)39-29(42)23-12-7-4-8-13-23)32(45)38-26(14-9-19-36-33(34)35)30(43)37-24-15-17-25(18-16-24)41(46)47;/h3-8,10-13,15-18,21,26-28H,9,14,19-20H2,1-2H3,(H,37,43)(H,38,45)(H,39,42)(H,40,44)(H4,34,35,36);1H/t26-,27-,28-;/m0./s1

InChI key

PYVSMZDQQUZGPF-JAQKLANPSA-N

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一般描述

N-苯甲酰基-Phe-Val-Arg-对硝基苯胺是显色蛋白酶底物。

应用

  • N

  • -苯甲酰基-Phe-Val-Arg-对硝基苯胺盐酸盐已作为底物：用于凝血酶菌丝
  • 的可溶性和颗粒部分中的胰蛋白酶样酶，来自蛇毒的重组和天然巴曲酶
  • 用于酰胺分解活性测定中的纤溶酶aprE2

包装

无底玻璃瓶。内含物装在插入的融合锥内。

底物

胰蛋白酶，凝血酶和蛇毒凝血酶的底物。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

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Methodological considerations on antithrombin determination.
U Abildgaard
Acta chirurgica Scandinavica. Supplementum, 509, 97-99 (1982-01-01)
Hydrolysis of N-alpha-benzoyl-L-phenylalanyl-L-valyl-L-arginine-p-nitroanilide by human alpha thrombin in the presence of heparin.
M J Griffith et al.
Thrombosis research, 17(1-2), 83-90 (1980-01-01)
The hydrolysis of N-benzoyl-L-phenylalanyl-L-valyl-L-arginine-p-nitroanilide and its use as a substrate for the assay of cathepsin B.
J Butterworth et al.
Analytical biochemistry, 106(1), 156-162 (1980-07-15)
Maxsuell Lucas Mendes Marques et al.
Marine drugs, 17(1) (2018-12-24)
In this study, sulfated polysaccharide-rich extracts were isolated from 22 tropical seaweeds (4 red, 11 brown, and 7 green) found in northeastern Brazil, and evaluated for the role of anticoagulant agents. Fifteen of the extracts showed anticoagulant activity, including all
P C Ring et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 30(8), 1085-1096 (2000-08-10)
Fel d 1, an important allergen from domestic cats, is a significant cause of asthma. In addition to directly promoting IgE synthesis, other biological activities of allergens may contribute to either allergic sensitization or the magnitude of allergic effector responses.
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实验方案

Thrombin from Bovine Plasma

Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

牛血浆凝血酶

凝血酶是一种内溶性丝氨酸蛋白酶，可选择性裂解纤维蛋白原的精氨酸-甘氨酸键，从而形成纤维蛋白并释放纤维蛋白肽A和B。

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