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应用
苯甲酰化萘酰化 DEAE-纤维素是一种用于蛋白质层析、离子交换层析和阴离子交换介质的纤维素介质。苯甲酰萘甲酰化 DEAE 特别适用于单链和双链 DNA 的分离。利用苯甲酰萘甲酰化 DEAE 设计了一种更有效的检测人乳头瘤病毒 DNA 的方法，并研究了药物 5-氟尿嘧啶 (FUra) 的细胞毒性。
制备说明
按 Gillam 等 生物化学 ，6, 3043 (1967) 方法制备。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
J D Schuetz et al.
Cancer chemotherapy and pharmacology, 21(3), 208-210 (1988-01-01)
5-Fluorouracil (FUra) was previously demonstrated to be incorporated into DNA at cytotoxic concentrations in mouse bone marrow cells. Subsequently, we showed that under these conditions FUra exhibited a time-dependent removal from DNA accompanied by a decrease in DNA strand length.
A N Koterov et al.
Biokhimiia (Moscow, Russia), 57(2), 195-200 (1992-02-01)
Single-stranded DNA-binding proteins (SSB-proteins) isolated from Ehrlich ascites tumour (EAT) cells were incubated for 30 min at 5 mM NaCl with salmon sperm DNA or [3H]DNA from EAT at the SSB-protein/DNA ratio (w/w) of 0 to 4.5. After addition of
R M Knobler et al.
Clinical and experimental dermatology, 17(6), 392-396 (1992-11-01)
Using the benzoylated naphthoylated DEAE cellulose method (BND-method) we have designed a more efficient approach for the detection of human papillomavirus-DNA (HPV-DNA) via dot-blot and hybridization. Biopsy material from anogenital warts (40 patients), invasive carcinoma uteri (12 patients) and normal
Michaela A Gold et al.
PLoS genetics, 17(10), e1009863-e1009863 (2021-10-22)
Disease-associated trinucleotide repeats form secondary DNA structures that interfere with replication and repair. Replication has been implicated as a mechanism that can cause repeat expansions and contractions. However, because structure-forming repeats are also replication barriers, it has been unclear whether
Ana Teixeira-Silva et al.
Nature communications, 8(1), 1982-1982 (2017-12-08)
Replication requires homologous recombination (HR) to stabilize and restart terminally arrested forks. HR-mediated fork processing requires single stranded DNA (ssDNA) gaps and not necessarily double strand breaks. We used genetic and molecular assays to investigate fork-resection and restart at dysfunctional
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